Abcam, ab316993, Human C3a anaphylatoxin Antibody Pair - BSA and Azide free

Size: 10 x 96Tests
Human C3a anaphylatoxin Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human C3a anaphylatoxin.
Key facts
Reacts with:Human,
Assay type:ELISA set,
Range:7.81 - 500 pg/mL,
Assay Platform:Reagents

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
Sandwich ELISA
FRET/TR-FRET/HTRF
Meso Scale Discovery
Bead-based assays
AlphaLISA
/AlphaScreen
DELFIA
immunoassays
and Single Molecule Counting (SMC™) immunoassays
Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody:
recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody:
recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration
: ~1 mg/ml
Storage buffer:
100% PBS
Liquid
Isotype:
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C3a anaphylatoxin also known simply as C3a functions as a small protein fragment within the complement system. It forms during the cleavage of complement component 3 (C3) and has a molecular mass of approximately 9 kDa. C3a is mainly expressed on leukocytes endothelial cells and smooth muscle cells. It acts as a potent mediator of inflammation by binding to the C3a receptor (C3aR) found on the surface of various immune cells like mast cells and basophils.
Biological function summary
C3a interacts extensively with cells involved in immune responses. It serves as an inflammatory mediator promoting chemotaxis mast cell degranulation and increased vascular permeability. C3a is not part of a large complex but acts independently to drive immune responses through its receptors. It also plays a role in the process of immune regulation and tissue repair by attracting immune cells to sites of infection or damage.
Pathways
C3a participates in both the complement and coagulation cascades. In the complement pathway it functions alongside other components like C5a and C5b-9 enhancing the local immune responses and pathogen clearance. The coagulation pathway also involves interactions with various other proteins affecting cell signaling and inflammation bridging innate and adaptive immunity.
C3a contributes to conditions like asthma and autoimmune disorders. In asthma it promotes airway inflammation and hyperresponsiveness by acting on immune cells and tissues. In systemic lupus erythematosus (SLE) C3a levels correlate with inflammation and tissue damage involving proteins in immune complex deposition. Understanding C3a's activity helps in developing therapeutic strategies targeting inflammatory diseases.