Abcam, ab317061, Alexa Fluor® 647 Anti-SIGLEC5 antibody [EPR26250-156]

Size: 100µL
Rabbit Recombinant Monoclonal SIGLEC5 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26250-156,
Isotype:IgG,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SIGLEC5 also known as CD170 is a sialic acid-binding Ig-like lectin with a molecular mass of approximately 96 kDa. This protein plays an important role in immune cell signaling by recognizing sialylated glycans on the surface of cells. SIGLEC5 is mainly expressed on immune cells such as neutrophils monocytes and macrophages. It belongs to the lectin family of proteins and can modulate immune responses and communication between cells.
Biological function summary
SIGLEC5 functions by mediating cell-cell interactions and transmitting inhibitory signals that regulate immune responses. It binds to sialic acid-containing glycoproteins which can help modulate the threshold for cell activation by transmitting signals through its immunoreceptor tyrosine-based inhibitory motif (ITIM). SIGLEC5 is not part of a large complex but interacts with other molecules to exert its function in maintaining immune cell balance.
Pathways
SIGLEC5 plays a role in immune signaling pathways by maintaining cell homeostasis. It contributes to the regulation of the immune response by modulating inhibitory signaling pathways. It is associated with proteins like Src family kinases which phosphorylate the ITIM motif to initiate downstream signaling cascades. Additionally its interaction with sialic acids allows modulation of the innate immune response to various stimuli.
SIGLEC5 has been linked to autoimmune diseases and infections like bacterial sepsis. In autoimmune conditions the dysregulation of SIGLEC5-associated pathways can lead to improper immune cell activation and tissue damage. In the context of bacterial sepsis SIGLEC5 assists in dampening excessive immune responses and limiting inflammation. SIGLEC5 connects with proteins like DAP12 where aberrations could also influence disease progressions making it a target of interest for potential therapeutic interventions.