Abcam, ab317146, Anti-Zika virus NS2B protein antibody [HL2151]

Size: 100µL
Rabbit Monoclonal POLG antibody. Suitable for ICC/IF, WB and reacts with Zika virus samples. Immunogen corresponding to Synthetic Peptide within Zika virus (isolate ZIKV/Human/French Polynesia/10087PF/2013) Zika virus NS2B protein.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:HL2151,
Isotype:IgG,
Carrier free:No,
Reacts with:Zika virus,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Zika virus (isolate ZIKV/Human/French Polynesia/10087PF/2013) Zika virus NS2B protein.A0A024B7W1

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Zika virus NS2B protein also known as ZIKV NS2B is a small non-structural protein with a molecular weight of approximately 14 kDa. This protein is part of the Zika virus polyprotein and becomes active through cleavage by viral proteases. NS2B acts as a cofactor enhancing the function of the viral NS3 protease which is important for viral replication. Expression of NS2B is predominantly in the cytoplasm of infected host cells where it plays a role in processing viral polyprotein into functional units necessary for the viral life cycle.
Biological function summary
NS2B provides an essential cofactor role within the viral replication machinery. It forms a complex with NS3 which possesses serine protease activity important for cleaving viral polyproteins. The NS2B-NS3 complex ensures efficient protein processing facilitating the synthesis of viral components. This interaction is vital for the replication of Zika virus within host cells and highlights the necessity of understanding NS2B's function in the Zika virus lifecycle.
Pathways
NS2B significantly impacts the viral replication process as part of the RNA-dependent RNA polymerase pathway. It partners with NS3 protease to process the polyprotein and aid in assembling the replication complex. NS2B also indirectly affects the host immune response pathways by influencing the processing and assembly of viral particles ensuring effective infection and replication within host cells. This role highlights its interplay with other proteins such as NS3 and NS5 all of which are necessary for viral success.
NS2B is intrinsically linked to Zika virus infection and related disorders like microcephaly and Guillain-Barré syndrome. The NS2B-NS3 protease complex serves as a potential target for antiviral drug development as inhibiting this interaction may prevent viral replication and reduce disease transmission. Understanding the interactions between NS2B NS3 and host proteins could provide insights into therapeutic strategies to combat Zika virus-related conditions.