Abcam, ab317155, Anti-WNK1 antibody [HL2184]

Size: 100µL
Rabbit Monoclonal WNK1 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human WNK1.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:HL2184,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human WNK1.Q9H4A3

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
WNK1 also known as With No Lysine 1 is a protein kinase with a molecular mass of approximately 251 kDa. Mechanically WNK1 functions as a serine/threonine kinase influencing the phosphorylation status of various substrates involved in ionic balance and cell volume regulation. It plays an important role in controlling ion transport channels. The protein is expressed in multiple tissues with high levels in the kidney and lower levels in the brain heart and other regions of the body. Its activity is especially important in the distal nephrons of the kidney.
Biological function summary
WNK1 affects sodium and potassium ion transport which is essential for maintaining homeostasis. It is a part of a larger signaling complex where it modulates the activity of SPAK and OSR1 kinases which directly affect ion transporters like NKCCs and NCC. By regulating these kinases WNK1 contributes to maintaining the balance of electrolytes influencing blood pressure and general cellular osmoregulation.
Pathways
WNK1 significantly impacts the WNK-SPAK/OSR1 signaling pathway which has direct roles in sodium and potassium ion transport. The pathway associates closely with proteins like SPAK and OSR1 which WNK1 activates affecting the function of ion transporters and channels. This signaling mechanism plays an integral role in renal salt reabsorption and is important for controlling systemic blood pressure.
Mutations and dysregulation of WNK1 link to hereditary hypertension and pseudohypoaldosteronism type II (PHAII) also known as Gordon's syndrome. These conditions involve elevated blood pressure and electrolyte imbalance due to altered transport activity. A change in WNK1 function can disrupt normal interaction with other proteins in its pathway such as NCC leading to these disorders. Understanding WNK1's role offers potential therapeutic targets for managing conditions related to electrolyte imbalance and blood pressure regulation.