Abcam, ab317175, Anti-Dengue virus NS3 protein antibody [HL2239]

Size: 100µL
Rabbit Monoclonal antibody. Suitable for WB and reacts with Transfected cell lysate - Dengue virus 2, Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4 samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:HL2239,
Isotype:IgG,
Carrier free:No,
Reacts with:Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Dengue Virus NS3 also known as NS3 protein is a non-structural protein with enzyme activity mainly acting as a serine protease and RNA helicase. Weighing approximately 69 kDa NS3 is expressed in infected cells of vertebrate hosts and is an important component in the virus replication process. It requires NS2B as a cofactor to display its protease activity facilitating the cleavage of the viral polyprotein precursor into functional proteins.
Biological function summary
NS3 protein plays a significant role in viral replication and maturation by partaking in the intracellular processes of the dengue virus. NS3 acts in concert with other non-structural proteins to ensure efficient replication of the viral genome. Particularly the helicase function assists in unwinding RNA during genome synthesis making it indispensable for the viral life cycle.
Pathways
NS3 protein participates in the dengue virus replication and maturation pathways which are critical for viral propagation. It interacts directly with other dengue virus proteins like NS2B and NS5 the latter functioning as the viral polymerase. NS3 and NS5 establish a multi-protein replicase complex that facilitates RNA genome replication and processing.
NS3 protein has a direct connection to dengue fever a widespread tropical disease caused by dengue virus infection. Its interaction with host immune proteins leads to pathogenic effects contributing to disease symptoms and progression. The NS3 protein's activity is a potential target for antiviral drug development aiming to inhibit its protease and helicase functions to reduce viral replication and mitigate disease severity.