Abcam, ab317188, Anti-Glutamine synthetase antibody [HL2283]

Size: 100µL
Rabbit Monoclonal Glutamine Synthetase antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate, Human, Mouse, Rat, Dog, Cat samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GLUL.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:HL2283,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat, Dog, Cat,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human GLUL.P15104

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glutamate-ammonia ligase also known as glutamine synthetase is an enzyme important for the synthesis of glutamine from glutamate and ammonia. This enzyme operates by catalyzing the ATP-dependent conversion playing a significant role in nitrogen metabolism. The human form of this enzyme has a mass of approximately 42 kDa. Glutamate-ammonia ligase is widely expressed found in tissues including the liver brain and kidneys pointing to its importance in diverse physiological processes.
Biological function summary
Glutamate-ammonia ligase performs an important function in nitrogen metabolism by catalyzing the reaction that produces glutamine a vital amino acid and a source of cellular carbon and nitrogen. This enzyme also participates in the detoxification of ammonia a process critical in the brain. It is known to operate as part of a larger enzyme complex working intricately with other cellular components to maintain nitrogen balance and detoxify harmful metabolic byproducts.
Pathways
The glutamate-ammonia ligase enzyme plays an integral role in the urea cycle and the glutamine-glutamate cycle. The urea cycle is pivotal in converting potentially harmful ammonia to urea for safe excretion showing close interaction with carbamoyl phosphate synthetase. In the glutamine-glutamate cycle the enzyme assists in neurotransmitter cycling and nitrogen metabolism interacting with proteins like glutaminase which converts glutamine back to glutamate.
Glutamate-ammonia ligase involvement links to conditions such as hepatic encephalopathy and neurodegenerative diseases like Alzheimer's disease. In hepatic encephalopathy enzyme dysfunction leads to impaired ammonia detoxification contributing to the neurological symptoms associated with liver disease. In neurodegenerative contexts altered glutamine and glutamate metabolism involve proteins such as amyloid precursor protein highlighting the enzyme's influence on brain health and disease progression.