Abcam, ab317352, Anti-FRS2 + FRS3 antibody [EPR28555-60] - BSA and Azide free (Detector)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal FRS2 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28555-60,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab317352 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify FRS2. The recommended pair for sandwich ELISA is:
Capture:
ab317351
, FRS2 Capture Antibody (unconjugated)
Detector: ab317352, FRS2 + FSR3 Detector Antibody (unconjugated)
The reference range value is 19.53-2500 pg/mL.
The recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The FRS2 and FRS3 proteins also known as Fibroblast Growth Factor Receptor Substrate 2 and 3 serve as adaptor proteins in signal transduction processes. FRS2 with a mass of approximately 60 kDa and FRS3 play key roles in linking cell surface receptors to downstream signaling pathways. They are ubiquitously expressed but have higher concentrations in certain tissues like the brain and heart. These proteins have modular structures that contain phosphotyrosine-binding domains and SH2 domains which are involved in protein-protein interactions.
Biological function summary
The adaptor proteins FRS2 and FRS3 mediate signals from receptor tyrosine kinases notably the Fibroblast Growth Factor Receptors (FGFRs). Through their action they facilitate the assembly of signaling complexes necessary for cellular proliferation differentiation and survival. FRS2 and FRS3 activate important downstream effectors like the Ras/MAPK and PI3K/AKT pathways. They not only recognize activated receptors but also recruit other adaptors and signaling molecules to form complex networks.
Pathways
The proteins FRS2 and FRS3 are integral to the FGFR and ERK/MAPK signaling pathways. In these pathways they are involved in transmitting external growth signals to the cell's interior leading to varied biological responses. They interact with other pathway components including GRB2 and SOS which further propagate the signal cascade. This places FRS2 and FRS3 at a central point in controlling cellular responses to external stimuli.
Mutations or malfunctions in FRS2 and FRS3 have been implicated in several conditions notably cancer and developmental disorders. Aberrant signaling through these adaptors can lead to uncontrolled cell proliferation contributing to oncogenesis. Additionally they have connections to developmental defects where improper signaling affects organogenesis and tissue formation. These proteins interact with key components such as FGFRs which are known to be altered in these disease states further linking FRS2 and FRS3 to the pathological processes.