Abcam, ab317739, Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal CHTB antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP, I-ELISA and reacts with Transfected cell lysate - Vibrio cholerae O1 biovar El Tor str. N16961, Transfected cell line - Vibrio cholerae O1 biovar El Tor str. N16961, Recombinant fragment - Vibrio cholerae O1 biovar El Tor str. N16961 samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27372-4,
Isotype:IgG,
Carrier free:Yes,
Applications:Flow Cyt (Intra), IHC-P, IP, I-ELISA, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab317739 is the carrier-free version of
ab317738
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cholera enterotoxin subunit B also known as CTB is the non-toxic component of cholera toxin with a molecular weight of approximately 11.6 kDa. CTB plays an important role in the toxin's ability to bind to target cells. Structurally CTB forms a pentameric ring that tightly binds to the GM1 ganglioside receptor on the surface of intestinal epithelial cells. While the CTB itself displays non-pathogenicity it facilitates the delivery of the toxic subunit causing the internalization of the entire cholera toxin complex. CTB is mainly expressed by the bacterium Vibrio cholerae which is found in aquatic environments especially in regions with poor sanitation.
Biological function summary
Cholera enterotoxin subunit B serves as a binding agent in the cholera toxin complex. Once CTB binds to the GM1 ganglioside receptors on intestinal epithelial cells it assists the A subunit also part of the cholera toxin complex in gaining entry into cells. Upon entry the A subunit triggers a cascade of events that results in dysregulation of cAMP levels leading to massive chloride ion secretion into the intestinal lumen. As a part of the entire cholera toxin CTB indirectly contributes to the excessive watery diarrhea that characterizes cholera infection.
Pathways
Cholera enterotoxin subunit B interacts with signal transduction processes related to cyclic AMP (cAMP) pathways. After CTB-mediated internalization the A subunit activates adenylate cyclase leading to increased cAMP in intestinal epithelial cells. This activation is an important event in the signaling pathway that causes altered ion transport across the cell membrane. This pathway is linked to the CFTR protein a chloride channel regulated by cAMP which plays a critical role in maintaining ion balance in epithelial tissues. This pathway emphasizes the connection between CTB and ion transport dysregulation in cholera.
Cholera enterotoxin subunit B is associated with cholera an acute diarrheal illness caused by Vibrio cholerae. CTB's role in facilitating the cholera toxin's effects links it indirectly to dehydration and electrolyte imbalance the main clinical manifestations of the disease. While CTB itself shows potential in therapeutic applications due to its non-toxic nature in context of Vibrio cholerae infection it remains an important player in the disease's pathogenesis. Interactions between CTB GM1 ganglioside and the CFTR protein underline its relevance in the disorder's mechanism.