Abcam, ab317745, Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal GPX8 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human, Rat, Mouse, Transfected cell lysate - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28823-80,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:ICC/IF, IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Unsuitable for mouse ICC

Product details:
ab317745 is the carrier-free version of
ab317744
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GPX8 also known as Glutathione Peroxidase 8 is an enzyme important in cellular antioxidant defense reducing hydrogen peroxide to water. It has a molecular mass of approximately 28 kDa. GPX8 localizes prominently within the endoplasmic reticulum membrane contributing to cellular redox homeostasis. This enzyme expresses in various tissue types with significant levels observed in the liver and kidney indicating its role in detoxifying reactive oxygen species.
Biological function summary
This enzyme plays an important role in maintaining cellular oxidative balance. It does not form part of a large complex but associates with other proteins in a network that mitigates oxidative damage. By catalyzing the reduction of peroxides it protects cellular components from oxidative stress-induced damage. GPX8's activity in reducing lipid peroxides underlines its importance in maintaining cellular membrane integrity particularly under stress conditions.
Pathways
GPX8 integrates into the cellular antioxidant defense system and the redox signaling pathways. It significantly contributes to the Glutathione metabolism pathway partnering with other GPX family members like GPX1. In the context of redox signaling it interacts indirectly with proteins influencing cellular responses to oxidative stress. These interactions facilitate the modulation of signaling cascades important for maintaining cellular homeostasis under oxidative conditions.
GPX8 is associated with oxidative stress-related conditions such as cancer and neurodegenerative diseases. For instance aberrant expression or activity can correlate with increased susceptibility to Hepatocellular Carcinoma where reactive oxygen species play a pivotal role. In neurodegenerative contexts this protein might interact with misfolded protein aggregates potentially implicating pathways linked to Parkinson's disease. As part of this disorder's complex oxidative stress mechanisms GPX8 could interact with proteins like Parkinson-associated DJ-1 highlighting a pathophysiological connection to the disease.