Abcam, ab317810, Anti-Bid antibody [EPR27444-102] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal BID antibody. Carrier free. Suitable for WB and reacts with Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27444-102,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab317810 is the carrier-free version of
ab317809
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Bid also known as BH3-interacting domain death agonist or BID protein functions as a pro-apoptotic member of the Bcl-2 protein family. This protein plays a significant role in the apoptotic process by helping to mediate the release of cytochrome c from mitochondria an important step in this cell death pathway. Bid exists in cells as an inactive uncleaved form but upon activation a caspase cleaves it into a truncated form tBID. The molecular weight of Bid is approximately 22 kDa. It is commonly expressed in numerous tissues throughout the body contributing to its various apoptotic regulatory roles.
Biological function summary
Bid induces mitochondria-mediated apoptosis that significantly affects cellular homeostasis. Bid is not considered to be part of a permanent protein complex; instead it acts as a mobile activator bridging interactions between different proteins within the apoptotic pathway. Upon activation tBID interacts with other Bcl-2 family members like Bax and Bak which leads to mitochondrial membrane permeabilization and subsequent release of cytochrome c.
Pathways
Bid significantly influences apoptotic signaling under different cellular stress conditions. It plays important functions in both intrinsic and extrinsic pathways of apoptosis. Within the intrinsic pathway Bid links the external death receptor signals to the mitochondria-mediated apoptotic pathway. In addition to interacting with Bax and Bak Bid indirectly connects to the apoptosome complex facilitating caspase activation which ultimately results in cellular apoptosis.
Bid has strong associations with conditions characterized by deregulated apoptosis such as cancer and neurodegenerative diseases. For instance downregulation or dysfunction of Bid is noted in certain cancer types contributing to the evasion of apoptosis a hallmark of cancer. Moreover in neurodegenerative diseases where excess apoptosis leads to loss of neuronal cells Bid activity has shown to connect with the regulation of apoptosis through its interactions with key apoptotic proteins such as Bcl-2 and Bax. Understanding Bid's role provides potential therapeutic targets to either promote or inhibit apoptotic processes in disease treatment.