Abcam, ab318168, APC Anti-SIGLEC8 antibody [EPR24419-56]

Size: 100µL
Rabbit Recombinant Monoclonal SIGLEC8 antibody - conjugated to APC. Suitable for Flow Cyt (Intra) and reacts with Transfected cell line - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24419-56,
Isotype:IgG,
Conjugation:APC,
Excitation/Emission:Ex: 650nm, Em: 660nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SIGLEC8 also known as Sialic acid-binding Ig-like lectin 8 is a protein that functions primarily as a receptor. It has a molecular mass of approximately 50 kDa. SIGLEC8 is expressed on the surface of certain immune cells specifically eosinophils basophils and to a lesser extent mast cells. This receptor plays an important role in the immune responses especially those involving inflammatory processes due to its ability to recognize and bind sialylated glycan ligands.
Biological function summary
SIGLEC8 serves as an inhibitory receptor on these immune cells which can help regulate their activation and survival. It does not form part of a large protein complex but its action can affect various cellular functions such as cell apoptosis and signaling processes. By binding to its specific glycan ligands SIGLEC8 reduces the lifespan of eosinophils which are often involved in allergic responses and asthma thereby helping to control inflammatory responses in tissues.
Pathways
SIGLEC8 is involved in pathways that regulate the immune system particularly those controlling eosinophil apoptosis and inflammation. It interacts with signaling pathways that include the Src family kinases which are central to signal transduction in immune cells. Additionally it shares functional relationships with other receptors in the SIGLEC family which modulate immune responses through similar mechanisms.
SIGLEC8 is significantly associated with asthma and other allergic diseases. The protein's ability to induce apoptosis in eosinophils makes it an important target for therapies aimed at controlling eosinophil-mediated inflammation in asthma. It is also related to the IL-5 signaling pathway as IL-5 plays a role in the survival and activation of eosinophils. By influencing these pathways SIGLEC8 holds potential as a therapeutic target for reducing symptoms and exacerbations in allergic conditions.