Abcam, ab318979, Anti-LDB1 antibody [EPR28912-75] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal LDB1 antibody. Carrier free. Suitable for Dot, IHC-P, ICC/IF, WB, IP, Flow Cyt (Intra) and reacts with Synthetic peptide - Human, Mouse, Rat, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28912-75,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Dot, IHC-P, WB, IP, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Not suitable for human IHC-P.

Product details:
ab318979 is the carrier-free version of
ab318978
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LDB1 also known as LIM Domain Binding 1 serves an important role in transcriptional regulation. This protein with a mass of approximately 42 kDa frequently expresses in tissues such as the brain heart and spleen. LDB1 participates in forming multiprotein complexes through its interaction with LIM homeodomain proteins engaging with various co-regulators to influence gene expression.
Biological function summary
LIM Domain Binding 1 plays an important role in developmental processes and cell differentiation. It combines with other proteins to form a LIM complex that acts as a DNA-binding transcriptional regulator. The complex binds to enhancer elements and facilitates the activation or repression of genes critical for the development of the nervous system and cardiovascular system.
Pathways
LIM Domain Binding 1 integrates into the Wnt signaling pathway and the TGF-beta pathway. It interacts with proteins such as TCF/LEF and Smad serving as a cofactor that modulates transcriptional responses and influences cellular outcomes. By participating in these pathways LDB1 helps regulate cell fate decisions ensuring appropriate cellular differentiation and proliferation responses.
LIM Domain Binding 1 holds relevance to leukemia and Wilms' tumor. Its dysregulation connects with disproportionate expression of key developmental genes contributing to pathogenesis. LDB1's interaction with transcription factors like SCL/TAL1 highlights its contribution to the onset of these diseases by modulating gene expression patterns associated with these cancers.