Abcam, ab319148, Anti-NUAK1/ARK5 antibody [EPR28626-53]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal ARK5 antibody. Suitable for Dot, WB and reacts with Recombinant fragment - Human, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28626-53,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:Dot, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody does not cross-react with human NUAK2 by dot blot.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NUAK1 also known as ARK5 is a protein kinase with an approximate molecular mass of 72 kDa. It belongs to the AMP-activated protein kinase (AMPK) family. This kinase is expressed in various tissues including the brain liver and skeletal muscle. NUAK1 plays a mechanical role by phosphorylating substrates which leads to modulation of their activity and localization. It interacts with cellular structures by influencing cytoskeletal dynamics cell adhesion and polarity.
Biological function summary
NUAK1 influences cell survival proliferation and metabolism by interacting with other proteins. It is not part of a stable complex but its activity affects many cellular processes. NUAK1 has a part in cellular responses to metabolic stress which helps cells to maintain energy homeostasis. By regulating processes such as apoptosis and autophagy NUAK1 impacts critical cellular functions.
Pathways
NUAK1 participates in the LKB1-AMPK signaling pathway. It acts downstream of LKB1 a tumor suppressor that activates AMPK-related kinases. This pathway is central to energy balance and metabolic control linking to cellular metabolism through substrates like ACC and mTOR. NUAK1 also has interactions with protein kinases like PKA through related signaling which further integrates its functions across cellular processes.
NUAK1 associates with cancer and metabolic disorders. In cancer its overactivity may drive tumor growth and metastasis by promoting cell survival pathways. NUAK1 has links with proteins such as MYC and Cyclin D which are important for cell cycle regulation. In metabolic disorders abnormalities in NUAK1 signaling can affect glucose uptake and insulin sensitivity associating it with conditions like type 2 diabetes. These findings make NUAK1 an interesting target for therapeutic research in these diseases.