Abcam, ab319150, Anti-PMCA2 antibody [EPR28923-56]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal Calcium Pump PMCA2 ATPase antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28923-56,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Not suitable for human IHC-P and rat ICC. This antibody weakly cross-react with PMCA1, PMCA3 and PMCA4 overexpression lysates.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Calcium Pump PMCA2 ATPase also known as PMCA2 or Ca-ATPase pump plays an important role in the active transport of calcium ions from the cytoplasm across the plasma membrane. It functions by hydrolyzing ATP to provide the energy needed for this process. PMCA2 belongs to the family of P-type ATPases and has a molecular mass of approximately 135 kDa. This pump is prominently expressed in the brain and inner ear particularly in the cerebellum and cochlea highlighting its importance in neuronal and auditory functions.
Biological function summary
PMCA2 regulates intracellular calcium concentration which is critical for cellular signaling and homeostasis. It does not form part of a larger complex but works independently to balance calcium levels. In neuronal cells efficient calcium clearance is necessary for synaptic activity and PMCA2 helps in maintaining the rapid calcium ion flux during neuronal firing. The presence of PMCA2 in auditory hair cells signifies its role in cochlear function and hearing sensitivity by aiding the processing of sound signals through calcium modulation.
Pathways
PMCA2 integrates into calcium signaling pathways and interacts with calmodulin which enhances its activity. Its function in the calmodulin-calcium signaling pathway is essential for processes such as neurotransmitter release and muscle contraction. In association with proteins like calmodulin PMCA2 ensures precise calcium dynamics safeguarding against cytotoxicity triggered by excessive calcium accumulation. The coordination with calmodulin formulates a pivotal connection within various signaling cascades.
PMCA2 mutations or dysfunctions have associations with neurological disorders and hearing impairments. Research links PMCA2 dysregulation to conditions such as spinocerebellar ataxia and hereditary hearing loss. In these scenarios PMCA2 interacts with molecular components that when impaired disrupt the normal calcium homeostasis. Such interruptions can lead to neurodegeneration or compromised auditory function underlining the significance of PMCA2 in maintaining health and preventing disease.