Abcam, ab320047, Mouse ALCAM (CD166) ELISA Kit- Extracellular

Size: 1 x 96Tests / 1 x 384Tests / 10 x 96Tests
Mouse ALCAM (CD166) ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Mouse ALCAM (CD166) with a sensitivity of 9.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Key facts
Detection method:Colorimetric,
Sample types:Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 9.2 pg/mL,
Range:28.125 - 1800 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse ALCAM (CD166) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ALCAM (CD166) protein in Serum, Cit plasma, EDTA Plasma, Hep Plasma, and Cell culture media. Quantitate Mouse ALCAM (CD166) with 9.2 pg/ml sensitivity.
SimpleStep ELISA
technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details. Our SimpleStep ELISA
technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD166 also known as ALCAM (activated leukocyte cell adhesion molecule) is a cell surface glycoprotein with a mass of approximately 105 kDa. This protein is widely expressed on various cell types including activated T-cells keratinocytes neurons and endothelial cells. In the immune system CD166 has roles in T-cell activation and migration. It contains five extracellular immunoglobulin-like domains which facilitate cell-cell interactions. Researchers often regard ALCAM-Z as an alternative form sharing structural elements with CD166 protein.
Biological function summary
CD166 mediates cell-cell adhesion and plays roles in neuronal development. It participates in the formation of synaptic junctions and affects neuronal differentiation. CD166 is also a component of cell signaling complexes influencing cell proliferation and migration. In addition it controls interactions between T-cells and dendritic cells impacting immune response. Its interactions with other adhesion molecules contribute to cellular communication and tissue integrity.
Pathways
CD166 functions within signaling pathways related to cell adhesion and migration. It is involved in the Wnt signaling pathway and its interactions regulate cell polarity and morphogenesis. CD166 interacts closely with proteins such as CD6 and Catenins impacting cellular signaling and the organization of the cytoskeleton. These pathways affect processes like embryonic development and immune cell function.
CD166 has implications in cancer and autoimmune diseases. Its dysregulation links to cancer metastasis where altered expression facilitates tumor invasion and spread. CD166 also relates to autoimmune diseases through its role in T-cell activation potentially contributing to conditions like multiple sclerosis. The interaction between CD166 and CD6 influences immune cell activation and may offer therapeutic targets for modulating immune responses in these disorders.