Abcam, ab320369, Alexa Fluor® 647 Anti-Munc 13-4 antibody [EPR4914]

Size: 100µL
Rabbit Recombinant Monoclonal Munc 13-4 antibody - conjugated to Alexa Fluor® 647.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4914,
Isotype:IgG,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Carrier free:No,
Applications:Target Binding Affinity, Antibody LabellingSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Munc13-4 also known as UNC13D acts as a critical mediator in the fusion of vesicles particularly in the exocytosis of cytotoxic granules in immune cells. This protein weighs approximately 120 kDa and resides in various immune cell types such as cytotoxic T lymphocytes and natural killer cells. Munc13-4 exists in the cytosol and relocates to the cell membrane upon activation supporting its role in exocytosis.
Biological function summary
Munc13-4 serves an essential function in immune response by facilitating granule-mediated cytotoxicity. This protein does not operate alone; it acts within a complex network with other members of the Unc13 protein family. The synergy between these proteins ensures the precise docking and priming of secretory vesicles aiding in effective immune responses against infected or malignant cells.
Pathways
Munc13-4 participates in the granule exocytosis pathway a central component of immune system functionality. Within this pathway Munc13-4 acts alongside proteins such as Syntaxin 11 and Rab27a influencing the regulation and release of cytolytic granules. Another vital pathway involving Munc13-4 is the calcium-dependent signaling pathway which modulates its activity and highlights its exocytotic role.
Defects in Munc13-4 have a strong association with familial hemophagocytic lymphohistiocytosis type 3 (FHL3) a severe immunological disorder. The impaired function of Munc13-4 results in defective cytotoxic granule exocytosis leading to improper immune regulation. Additionally connections exist between Munc13-4 dysfunction and systemic lupus erythematosus (SLE) which implicates proteins such as Perforin and Rab27a in altered immune responses.