Abcam, ab320767, Anti-ADAM22 antibody [EPR28020-51] - BSA and Azide free (Detector)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ADAM22 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28020-51,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab320767 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human ADAM22. The recommended pair for sandwich ELISA is:
Capture:
ab320766
, Human ADAM22 Capture Antibody (unconjugated)
Detector: ab320767, Human ADAM22 Detector Antibody (unconjugated)
The reference range value is 125-8000 pg/mL.
The recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ADAM22 also known as ADAM 22 or ADAM 2 is a protein that functions mechanically as a transmembrane receptor involved in cell-cell and cell-matrix interactions. This protein does not exhibit protease activity unlike other members of the ADAM family which distinguishes its functional role. ADAM22 has a mass of approximately 100 kDa. It is expressed predominantly in the central nervous system particularly in brain tissues where it plays a significant role in neuronal signaling.
Biological function summary
ADAM22 affects the nervous system's development and function by mediating synaptic formation and maintenance. It belongs to a protein complex with LGI1 which also interacts with other proteins on the synaptic membrane facilitating communication between neurons. The LGI1-ADAM22 complex reportedly regulates ion channels and other synaptic proteins impacting neurotransmission and synaptic plasticity essential for cognitive processes.
Pathways
ADAM22 acts within the neuropharmacological and synaptogenesis pathways. It interacts closely with LGI1 and PSD95 pivotal proteins in developing synaptic signaling platforms. These pathways influence neurotransmitter release and receptor clustering fundamental for effective neural circuit function. The disruption of this pathway or its elements can lead to neurological impairments and highlight ADAM22's role in maintaining neural integrity.
ADAM22 connects to neurological conditions such as epilepsy and glioma. Mutations or deficiencies in the LGI1-ADAM22 interaction have been linked to epilepsy affecting ion channel regulatory actions. In glioma altered expression of ADAM22 may play a role in tumor progression influenced by interactions with integrins and growth factors. Therefore ADAM22's presence and regulatory actions contribute to neurological and oncological disease models.