Abcam, ab321971, Anti-TMEM106B antibody [EPR27525-70] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal T106B antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Recombinant fragment - Human, Human, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27525-70,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Unsuitable for mouse ICC. This antibody cross-react with recombinant human TMEM106C by western blot.

Product details:
ab321971 is the carrier-free version of
ab321970
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TMEM106B also known as transmembrane protein 106B is a type II single-pass transmembrane protein with a molecular mass of approximately 33 kDa. It is expressed in specific regions of the brain as well as in other tissue types such as the lungs and immune system cells. Its expression levels are especially elevated in microglia and oligodendrocytes which are brain cells that play important roles in immune response and myelination respectively. Structurally TMEM106B contains a transmembrane domain and its precise biochemical function is still under investigation though it is thought to participate in lysosomal and vesicular trafficking.
Biological function summary
TMEM106B is involved in several cellular processes impacting lysosome function and trafficking. It may become part of a protein complex that manages lysosomal positioning and pathway regulation within the cell. TMEM106B also affects cellular homeostasis and protein degradation which suggests a role in maintaining the proper balance of proteins and lipids within lysosomes. Alteration in TMEM106B expression or function can cause changes in lysosomal size and distribution indicating its role in cellular logistics.
Pathways
The TMEM106B protein becomes involved in the endolysosomal pathway and the autophagy-lysosome pathway. These pathways are critical for processing and breakdown of cellular waste and recycling of cellular components. In these pathways TMEM106B interacts with other proteins such as SORT1 (sortilin 1) and GRN (progranulin) both of which have important roles in protein sorting and trafficking. By influencing these pathways TMEM106B helps maintain cellular health and function.
TMEM106B links to neurodegenerative conditions such as frontotemporal lobar degeneration and possibly Alzheimer's disease. Mutations or abnormal expression levels of TMEM106B have been implicated in the pathogenesis and progression of these diseases. In frontotemporal lobar degeneration TMEM106B interacts with proteins like GRN where changes can lead to neurodegeneration. In Alzheimer's disease abnormal lysosomal function tied to TMEM106B may contribute to the accumulation of pathogenic proteins.