Abcam, ab322048, Anti-GLEPP1/PTPRO antibody [EPR28645-553] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal GLEPP1/PTPRO antibody. Carrier free. Suitable for Dot, IHC-P, mIHC and reacts with Recombinant fragment - Human, Human, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28645-553,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse,
Applications:Dot, IHC-P, mIHCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab322048 is the carrier-free version of
ab322047
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GLEPP1/PTPRO also known as protein tyrosine phosphatase receptor type O is a receptor-type protein tyrosine phosphatase with a mass around 155 kDa. It is mainly expressed in epithelial cells like podocytes in the kidney glomerulus neurons in the brain and other tissues like liver and lung. GLEPP1/PTPRO catalyzes the removal of phosphate groups from tyrosine residues on proteins an action important for regulating protein function and signal transduction. In podocytes its activity helps maintain the filtration barrier of the kidney.
Biological function summary
GLEPP1/PTPRO participates in cellular growth differentiation and apoptosis. It forms part of the podocyte slit diaphragm complex important for maintaining the glomerular filtration function in kidneys. This protein also interacts with other cellular proteins to regulate signaling pathways that control cellular architecture. Its enzymatic activity influences cellular processes by altering the phosphorylation status of partner proteins.
Pathways
GLEPP1/PTPRO plays essential roles in the MAPK and JAK/STAT signaling pathways. Through these pathways the protein mediates cellular responses to external signals influencing processes like cell cycle progression and survival. It interacts with proteins like Jak2 in these pathways modulating their activities to maintain cellular homeostasis. This involvement emphasizes its importance in signaling impacting cellular responses to environmental changes.
GLEPP1/PTPRO is linked to kidney diseases such as focal segmental glomerulosclerosis (FSGS) and certain cancers. Its dysregulation can lead to abnormal podocyte function contributing to glomerular disease. Moreover in cancer alterations in its activity might affect cancer cell behavior promoting tumor progression. The relation of GLEPP1/PTPRO with proteins like nephrin highlights its contribution to maintaining healthy kidney function and its potential role in disease mechanisms.