Abcam, ab322089, Recombinant Mouse AGT Protein Standard (His tag)

Size: 10µg
Recombinant Mouse AGT Protein Standard (His tag) is a Mouse Full Length protein, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, sELISA.
Key facts
Purity:>80% SDS-PAGE,
Expression system:HEK 293 cells,
Tags:His tag C-Terminus,
Applications:SDS-PAGE, sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P11859,
Animal free:Yes,
Carrier free:No,
Species:Mouse,
Storage buffer:pH: 7.3 - 7.5Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

Product details:
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our
Scientific Support
team to know which antibody pair is suitable for this protein.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Angiotensin is a peptide hormone critical in regulating blood pressure and fluid balance. Often referred to as Angiotensin I II or III depending on its form this target has a variable mass depending on the specific peptide variant. It primarily expresses in the liver where it is originally synthesized as a precursor protein called angiotensinogen. This protein circulates in the bloodstream and is cleaved to form active peptides when needed. Angiotensin acts on vascular smooth muscles and adrenal glands playing a significant role in vasoconstriction and aldosterone secretion.
Biological function summary
The peptide interacts with specific receptors in different tissues to exert its effects. It is part of the renin-angiotensin system (RAS) where it forms a functional complex with enzymes such as renin and angiotensin-converting enzyme (ACE). This system includes various feedback mechanisms maintaining homeostasis within the cardiovascular and renal systems. Angiotensin II the most active form binds to receptors on blood vessels increasing their constriction and therefore raising blood pressure. It also influences sodium and water retention through its action on the kidneys.
Pathways
This peptide fits into the larger framework of the RAS which is important for the regulation of vascular resistance. Angiotensin interacts directly with angiotensinogen which renin processes to form Angiotensin I. Subsequently ACE converts Angiotensin I to Angiotensin II. This cascade affects several downstream pathways influencing cardiovascular function and fluid retention. By impacting other proteins involved in these pathways such as aldosterone it plays an integral role in circulatory and renal physiology.
Angiotensin is closely linked to hypertension and heart failure. Dysregulation of the renin-angiotensin system or overproduction of Angiotensin II often leads to elevated blood pressure contributing to these conditions. Angiotensin receptor blockers (ARBs) and ACE inhibitors serve as therapeutic interventions in these diseases. Moreover in the case of heart failure excessive activity of Angiotensin can worsen the condition by increasing cardiac workload. It interacts with other proteins like ACE in these disorders forming a target for therapeutic modulation to improve patient outcomes.