Abcam, ab322090, Recombinant Human P-Selectin Protein Standard (His tag)

Size: 10µg
Recombinant Human P-Selectin Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, sELISA.
Key facts
Purity:>80% SDS-PAGE,
Expression system:HEK 293 cells,
Tags:His tag C-Terminus,
Applications:SDS-PAGE, sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P16109,
Animal free:Yes,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.3 - 7.5Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

Product details:
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our
Scientific Support
team to know which antibody pair is suitable for this protein.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
P-Selectin also known as CD62P or GMP-140 is a cell adhesion molecule expressed on the surface of activated endothelial cells and platelets. It has a molecular weight of approximately 140 kDa. In resting cells P-Selectin resides within alpha granules of platelets and Weibel-Palade bodies of endothelial cells. Upon activation it rapidly translocates to the cell surface playing an important role in mediating the initial interactions between endothelial cells leukocytes and platelets by binding to its ligand P-Selectin glycoprotein ligand-1 (PSGL-1).
Biological function summary
P-Selectin facilitates the recruitment of leukocytes to the site of injury or inflammation. It is a member of the selectin family of cell adhesion molecules which also includes E-Selectin and L-Selectin. P-Selectin interactions promote rolling and tethering of leukocytes on the endothelium a critical step for leukocyte extravasation into tissues. These processes are important for innate immune responses and the inflammation process.
Pathways
P-Selectin acts within the coagulation and inflammation pathways. In the coagulation pathway P-Selectin enhances platelet aggregation by interacting with fibrin and promoting thrombus formation. In the inflammation pathway it interacts with chemokines and integrins such as ICAM-1 facilitating leukocyte adhesion and transmigration. By partnering with these molecules P-Selectin coordinates cellular events that lead to immune response and wound healing.
P-Selectin is implicated in atherosclerosis and thrombosis. In atherosclerosis excessive expression of P-Selectin contributes to the recruitment of monocytes to the arterial wall promoting foam cell formation and plaque growth. In thrombosis elevated P-Selectin levels enhance platelet-leukocyte interactions exacerbating thrombus formation. The protein VWF (von Willebrand factor) is closely related to P-Selectin in these conditions as it also promotes platelet adhesion and aggregation.