Abcam, ab32503, Anti-Bax antibody [E63]

Size: 20µL / 100µL / 1mL
Anti-Bax antibody [E63] (ab32503) is a rabbit monoclonal antibody detecting Bax in Western Blot, IP, IHC-P, ELISA . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 2470 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:E63,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human, Mouse,
Applications:IP, WB, sELISA, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Expression levels of BAX protein vary with sample type. Induction may be required if endogenous expression is low.

Product details:
Anti-Bax antibody [E63] (ab32503) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-P, IP, WB, sELISA in human, mouse, rat samples.
Anti-Bax antibody [E63] (ab32503) has been cited over 2479 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Bax antibody [E63] (ab32503) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-Bax antibody [E63] (ab32503) has been confirmed by testing in knockout samples.
Anti-Bax antibody [E63] (ab32503) has 12 independent reviews from customers.
Anti-Bax antibody [E63] (ab32503) specifically detects Bax (UniProt ID: Q07812; Molecular weight: 21kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone E63 -
ab216985
Antibody clone E63 is also available pre-conjugated to a variety of labels for your convenience - HRP (
ab205822
The BAX gene (BCL2 Associated X) is a key player in the regulation of apoptosis, or programmed cell death, which is crucial for maintaining cellular homeostasis and preventing cancer. BAX is part of the BCL-2 protein family and promotes apoptosis by increasing mitochondrial membrane permeability, leading to the release of cytochrome c and activation of caspases. In oncology, BAX expression is regulated by the tumor suppressor protein p53, which is often mutated in cancer. Mutations or dysregulation of BAX can contribute to cancer development and progression by allowing cancer cells to survive.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Shipping conditions update: ambient shipping
This product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality.
Why the change?
It’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene.
What you need to know
Ambient shipments come clearly marked on the delivery note.
No ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products.
Warranty coverage remains fully valid, aligned with our validated shipping method.
Please store the product as per the datasheet instructions upon receipt.
Find out more -
https://www.abcam.com/en-us/support/shipping-storage-support/ambient-shipping

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Conditional Ambient, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Bax also known as Bcl-2-associated X protein plays an important role in the regulation of apoptosis. It is widely known for its pro-apoptotic function opposing the action of anti-apoptotic proteins like Bcl-2. Bax has a molecular weight of approximately 21 kDa making it identifiable in laboratory techniques such as western blot. Researchers often use anti-Bax antibodies to detect and analyze its expression levels in various studies. The Bax protein is mainly expressed in mitochondria where it undergoes changes in response to apoptotic signals.
Biological function summary
The Bax protein forms homodimers or heterodimers with other Bcl-2 family proteins to facilitate apoptosis. This protein is part of a larger complex involving several members of the Bcl-2 family which finely tunes the balance between cell survival and programmed cell death. Through a change in its conformation Bax translocates to the outer mitochondrial membrane where it promotes the release of cytochrome c and other apoptogenic factors into the cytosol.
Pathways
Bax plays a significant role in the intrinsic pathway of apoptosis also known as the mitochondrial pathway. This pathway involves the release of cytochrome c which further activates downstream proteins such as caspase-9 and caspase-3. Bax interacts closely with proteins like Bak another pro-apoptotic member of the Bcl-2 family providing a cooperative function in mitochondrial membrane permeabilization. Together Bax and Bak act as gatekeepers of the mitochondrial pathway determining the cell's fate in response to apoptotic stimuli.
The dysregulation of Bax often associates with various cancers and neurodegenerative diseases. In cancer reduced Bax expression or function can lead to resistance to apoptosis contributing to unchecked cellular proliferation. Conversely in neurodegenerative disorders such as Alzheimer's disease increased Bax activity results in excessive neuronal apoptosis. The balance between Bax and its partner proteins like Bcl-2 or Bcl-xL is important in maintaining normal cellular function and preventing disease progression.