Abcam, ab33780, Anti-Glucose Transporter GLUT4 antibody

Size: 100µg
Anti-Glucose Transporter GLUT4 antibody (ab33780) is a rabbit polyclonal antibody detecting Glucose Transporter GLUT4 in IHC-P, ICC/IF . Suitable for Human, Mouse, . - Over 50 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

Product details:
Anti-Glucose Transporter GLUT4 antibody (ab33780) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P in human, mouse samples.
Anti-Glucose Transporter GLUT4 antibody (ab33780) has been cited over 57 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Glucose Transporter GLUT4 antibody (ab33780) has high sensitivity and specificity.
Anti-Glucose Transporter GLUT4 antibody (ab33780) has 10 independent reviews from customers.
Anti-Glucose Transporter GLUT4 antibody (SLC2A4 antibody) (ab33780) specifically detects Glucose Transporter GLUT4 (UniProt ID: P14672; Molecular weight: 55kDa) and is sold in 100 µg selling sizes.
GLUT4 (SLC2A4) is an essential insulin-regulated glucose transporter predominantly found in skeletal muscle and adipose tissue. Utilizing our SLC2A4 antibody can help to accurately detect and study of GLUT4 expression. Research indicates that GLUT4 plays a crucial role in maintaining glucose homeostasis, making it a vital target for understanding metabolic diseases such as type 2 diabetes.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glucose transporter GLUT4 also known as GLUT-4 or GLUT 4 transporter plays an important role in glucose uptake in cells. It is an insulin-responsive class I facilitated glucose transporter. GLUT4 predominantly expresses in insulin-sensitive tissues such as adipose tissue and skeletal and cardiac muscles. The functional transport mechanism facilitates glucose entry into the cell by translocating from intracellular vesicles to the plasma membrane in response to insulin stimulation. The molecular weight of GLUT4 is about 50 kDa and this property is often observed in techniques such as GLUT4 western blot where it aids in the identification of the transporter.
Biological function summary
GLUT4 helps regulate blood sugar levels through its control over glucose uptake in muscle and adipose tissues. As an important component of the insulin-regulated glucose transport system GLUT4 responds to increased insulin levels by moving to the cell's surface aiding in glucose transportation into the cell. This adaptive response reduces blood glucose concentration after food intake. GLUT4 operates largely independently but may interact with other glucose transporters like GLUT1 to maintain normal cellular glucose homeostasis.
Pathways
GLUT4 plays a significant role in the insulin signaling pathway and glucose homeostasis. Activation of the insulin receptor triggers a signaling cascade that includes enzymes like Akt (protein kinase B) which facilitates the translocation of GLUT4 vesicles to the plasma membrane. This translocation is important for glucose entry into the cells. Other key proteins in this pathway include IRS (insulin receptor substrate) proteins which act as signaling intermediates to link insulin receptor activation to downstream targets like GLUT4. Its activity is central to the maintenance of energy balance and storage in the body.
GLUT4 is closely associated with conditions like type 2 diabetes and obesity. Impaired GLUT4 function or expression can lead to decreased insulin sensitivity and glucose uptake contributing to the hyperglycemic state found in type 2 diabetes. Additionally obesity often presents a parallel decline in GLUT4 activity exacerbating insulin resistance. The regulation of GLUT4 is influenced by proteins such as insulin receptor and IRS proteins which when dysfunctional contribute to the pathogenesis of these metabolic disorders.