Abcam, ab3505, Anti-AANAT antibody

Size: 50µg
Rabbit Polyclonal AANAT antibody. Suitable for WB, IHC-P and reacts with Rat samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Mouse Aanat aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Mouse Aanat aa 1-50. The exact immunogen used to generate this antibody is proprietary information.O88816

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Total anti-N-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide. Phospho-specific amino-terminal IgG was subsequently isolated on resin-coupled phosphopeptide., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AANAT also known as aralkylamine N-acetyltransferase or serotonin N-acetyltransferase is an enzyme with a molecular mass of approximately 23.3 kDa. This enzyme catalyzes the transfer of an acetyl group from acetyl-CoA to serotonin forming N-acetylserotonin. AANAT expression is mainly found in the pineal gland where it acts as an important player in melatonin biosynthesis. The function of AANAT as a regulatory point in this biosynthesis contributes to the role of melatonin in circadian rhythms and sleep regulation.
Biological function summary
AANAT has a critical role in regulating physiological processes through the production of melatonin. It operates as part of the intramolecular complex that facilitates its enzymatic activity in the pineal gland. By converting serotonin into N-acetylserotonin which further converts to melatonin AANAT modulates the synthesis of melatonin an indoleamine with effects on sleep-wake cycles and seasonal reproductive rhythms in mammals. This regulatory activity reflects how AANAT influences the hormonal signaling pathways in response to light and dark cycles.
Pathways
AANAT is integral to the melatonin synthesis pathway and interacts closely in serotonin metabolism. It tightly regulates the conversion of serotonin to melatonin in the pineal gland especially during nocturnal periods. One key protein related to AANAT in this pathway is hydroxyindole O-methyltransferase (HIOMT) which converts N-acetylserotonin to melatonin. The modulation of melatonin levels impacts diverse biological pathways including circadian rhythm regulation and neuroendocrine signaling.
AANAT plays a significant role in conditions like seasonal affective disorder (SAD) and sleep disturbances. Alterations in AANAT expression or function can affect melatonin levels leading to these disorders. Additionally disruptions in the enzymatic activity of AANAT may have interactions with serotonin influencing its availability and potentially linking it to disorders like depression. Understanding AANAT's function allows researchers to explore therapeutic strategies targeting melatonin pathways in managing these conditions.