Abcam, ab3581, Anti-Glucocorticoid Receptor beta antibody

Size: 50µL
Rabbit Polyclonal Glucocorticoid Receptor antibody. Suitable for WB and reacts with Transfected cell lysate - Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human NR3C1 aa 700-750.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human NR3C1 aa 700-750. The exact immunogen used to generate this antibody is proprietary information.P04150

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glucocorticoid Receptor beta (GRβ) also known as NR3C1 isoform β is a variant of the glucocorticoid receptor. Unlike its counterpart GRα GRβ does not bind glucocorticoids due to differences in its ligand-binding domain. With an approximate mass of 90 kDa GRβ acts primarily as a dominant negative regulator of GRα. It is expressed in various tissues including skin lung and immune cells which suggests it has a wide-reaching influence on cellular responses to glucocorticoids.
Biological function summary
GRβ functions as a modulator of glucocorticoid signaling by inhibiting the activity of GRα. Though it does not bind glucocorticoids itself it can still form part of a receptor complex that affects transcriptional activities. By reducing GRα-mediated gene expression GRβ influences the body's response to stress and inflammation. Its role as a competitor to GRα shapes the regulation of genes involved in metabolism immune function and cellular growth.
Pathways
The regulatory role of GRβ in glucocorticoid signaling impacts the hypothalamic-pituitary-adrenal (HPA) axis which is important for stress response and homeostasis. GRβ interacts with proteins involved in the NF-kB pathway connecting it to the regulation of immune and inflammatory responses. Through its effect on these pathways GRβ exerts control over the transcriptional regulation shared with GRα and other transcription factors influencing cellular processes like apoptosis and autophagy.
Alterations in GRβ expression or function link to conditions such as corticosteroid resistance and autoimmune diseases including rheumatoid arthritis. GRβ's interaction with GRα is significant because imbalances in their ratios can alter the usual glucocorticoid effects contributing to disease pathology. GRβ also connects to proteins in inflammatory signaling cascades making it an important player in understanding the molecular mechanisms underlying resistance and sensitivity to glucocorticoid therapy.