Abcam, ab36392, Anti-Natural Killer Cell Activation Structures antibody [ANK61]

Size: 500µL
Mouse Monoclonal Natural Killer Cell Activation Structures antibody. Suitable for IP, Flow Cyt, IHC-P, IHC-Fr and reacts with Rat samples. Cited in 12 publications. Immunogen corresponding to Cell preparation containing Natural Killer Cell Activation Structures protein.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:ANK61,
Isotype:IgG1,
Carrier free:No,
Reacts with:Rat,
Applications:IP, Flow Cyt, IHC-Fr, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The antigen recognized by ab36392 is highly expressed on freshly isolated and cultured NK cells. The antibody also binds to rat alpha beta TCR T cells and at a low level to B cells.

Product details:
Triggering of the antigen by ab36392 antibody activates the lytic machinery of rat NK cells, but not of T cells.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.3Preservative: 0.05% Sodium azideConstituents: Tissue culture supernatant, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Natural Killer (NK) Cell Activation Structures are essential components responsible for triggering the killing activity of NK cells. These structures are primarily located on the surface of NK cells where they interact with ligands on target cells. The mass of these activation structures can vary as they often comprise clusters of proteins such as CD16 also known as Fc receptor IIIA and their associated molecules. CD16 has an estimated molecular weight of around 50 kDa. NK cell activation structures are expressed across various tissues where immune surveillance is necessary.
Biological function summary
These structures serve to initiate the cytotoxic response of NK cells by recognizing stressed cells and those lacking "self" markers. When NK cell activation structures engage target cells they form part of a complex that includes proteins such as NKG2D and NKp46. These complexes recognize specific ligands on potential target cells facilitating the release of cytotoxic granules and cytokines. This biological function ensures that NK cells can target and eliminate infected or cancerous cells while sparing normal cells.
Pathways
NK cell activation structures play an important role in the immune response pathways specifically the natural cytotoxicity pathway. They interact with signaling molecules like DAP10 and DAP12 which transmit activation signals to the interior of the NK cells. This signal transduction enhances the ability of NK cells to respond to infections and cancerous changes. Another pathway is the Fc receptor pathway in which CD16 is associated with the activation of NK cells through antibody-dependent cellular cytotoxicity (ADCC).
Activating structures of NK cells have connections to conditions such as cancer and autoimmune diseases. In cancer defective NK cell activation can lead to insufficient immune surveillance allowing tumor cells to proliferate. Malfunctions in these structures potentially linked with proteins like MHC class I chain-related proteins can also result in an autoimmune response where NK cells mistakenly target healthy tissue. Understanding and targeting these structures might lead to improved therapeutic strategies for modulating immune responses in these diseases.