Abcam, ab37477, Anti-CTCF antibody [mAbcam 37477]

Size: 100µg
Mouse Monoclonal CTCF antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:mAbcam 37477,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody has previously been successfully used in western blot after immunoprecipitation from mouse NIH3T3 lysates. Please see Yao H et al. Genes Dev 24:2543-55 (2010), PMID: 20966046 for more details.

Product details:
CTCF is known to migrate by SDS-PAGE at 130 kDa (PMID: 9016583) We can conjugate this antibody to FITC for you (please see
ab150242
for details).
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CTCF also known as the CCCTC-binding factor is a highly conserved zinc finger protein involved in transcriptional regulation and chromatin organization. It has a molecular weight of approximately 82 kDa. The CTCF protein plays a critical mechanical role in controlling the three-dimensional architecture of the genome by binding to specific DNA sequences and forming chromatin loops. It is widely expressed in various cell types across tissues where it acts as a transcriptional repressor and activator depending on the context. CTCF immunofluorescence techniques enable the visualization of its dynamic distribution and expression within the nucleus.
Biological function summary
The multipurpose CTCF protein acts in coordinating the spatial organization of the genome. It functions as an insulator by regulating the boundaries between different chromosomal domains and controlling gene expression. CTCF operates within various complexes interacting with cohesin a vital protein complex that facilitates loop formation and influences genome architecture. This interaction helps in maintaining the integrity of the genome structure and proper chromatin insulation which are essential for normal gene function.
Pathways
CTCF plays significant roles in epigenetic regulatory networks and transcriptional pathways. In the epigenetic landscape it influences gene expression through modulation of DNA methylation states at CpG islands interacting with proteins like DNA methyltransferases. In transcriptional pathways CTCF interacts with nuclear factor Y (NF-Y) which contributes to cell cycle regulation by modulating the expression of cell cycle genes. These pathways reflect CTCF's versatility in gene regulation and its influence on maintaining cellular homeostasis.
CTCF disruptions have been implicated in cancer and intellectual disabilities. Mutations or altered expression of CTCF can lead to tumorigenesis as CTCF acts as a tumor suppressor by controlling oncogene and tumor suppressor gene expression. In intellectual disabilities CTCF mutations affect brain development by disrupting the expression of neuronal genes. The protein's interaction with cohesin has links to disorders such as Cornelia de Lange syndrome where cohesin complex dysfunction parallels the phenotypes seen with CTCF aberrations.