Abcam, ab37484, Anti-Myosin heavy chain antibody [A4.1025]

Size: 20µg / 100µg / 1mg
Anti-Myosin heavy chain antibody [A4.1025] (ab37484) is a mouse monoclonal antibody detecting myosin heavy chains 1,2,3,4,5,6,7 and 8 in Western Blot , IHC-P , IHC-Fr , ICC/IF . Suitable for Human , Mouse , Rat . - Trusted since 2006
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:A4.1025,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, IHC-Fr, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:ATP binding loop of myosin heavy chain skeletal and cardiac muscle.

Product details:
Product Specifications
Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484) is a mouse recombinant monoclonal antibody and is validated for use in ICC/IF, IHC-Fr, IHC-P, WB in human, mouse, rat samples.
Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484) specifically detects Myosin (UniProt ID: P12882; Molecular weight: 223kDa) and is sold in 100 µg and 1 mg selling sizes.
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Related Products
Antibody clone A4.1025 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, Alexa Fluor
ab315962
ab315963
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Myosin is a molecular motor protein responsible for converting chemical energy into mechanical force facilitating muscle contraction and cellular movement. Also known as the myosin heavy chain (MHC) or MYH myosin has a commonly referenced heavy chain Myosin heavy chain 1 (MYH1) with a molecular mass of roughly 200 kDa. Myosin is broadly expressed in muscle tissues including skeletal cardiac and smooth muscles and appears in various isoforms depending on the specific type of muscle tissue.
Biological function summary
Myosin interacts intricately with actin filaments to form a complex that executes muscle contraction and cell motility. Myosin heads bind to actin filaments employing ATP hydrolysis to generate motion. This protein is a fundamental component of the sarcomere the structural unit of muscle fibers and works in concert with other proteins like tropomyosin and troponin to regulate muscle contraction.
Pathways
Myosin has a significant role in the muscle contraction and cell motility pathways. Myosin and actin interaction is central to the sliding filament theory which is an essential part of the muscle contraction pathway. Myosin is also involved in non-muscle cell movement through the actin-myosin contractile ring in the process of cytokinesis. Myosin interacts with proteins such as actin and ATP in these pathways to facilitate cellular processes.
Myosin has connections to conditions like hypertrophic cardiomyopathy and various myopathies. Mutations in the myosin heavy chain can lead to hypertrophic cardiomyopathy affecting the heart's ability to pump blood effectively. Additionally disruptions in myosin function can contribute to muscular diseases impairing muscle strength and function. Myosin's interaction with actin and related sarcomeric proteins is pivotal in these pathological scenarios often resulting in altered muscle dynamics and associated clinical manifestations.