Abcam, ab37916, Anti-p21-ARC/ARPC3 antibody

Size: 100µg
Goat Polyclonal p21-ARC/ARPC3 antibody. Suitable for WB and reacts with Mouse samples. Cited in 3 publications.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The p21-ARC also known as ARPC3 is a subunit of the actin-related protein 2/3 complex (Arp2/3 complex). This protein plays a critical role in the nucleation of new actin filaments promoting the formation of branched actin networks. ARPC3 has a molecular mass of approximately 21 kDa and its expression can be found in a variety of tissues including the heart and kidney. The protein operates in the cytoplasm where it associates with other proteins in the Arp2/3 complex to mediate its function.
Biological function summary
The p21-ARC/ARPC3 is an essential component of the highly conserved Arp2/3 complex involved in actin filament assembly and organization. This complex binds to the sides of pre-existing actin filaments and nucleates new ones creating branching and forming web-like structures needed for various cellular processes. The Arp2/3 complex including ARPC3 is involved in cell movement endocytosis and intracellular trafficking. This protein's activity enhances the dynamic restructuring of the actin cytoskeleton necessary for these functions.
Pathways
ARPC3 influences pathways related to cell motility and cytoskeletal reorganization. It participates in the Wiskott-Aldrich Syndrome Protein (WASP) pathway where it acts alongside proteins such as WASP and the WAVE complex to regulate actin polymerization. The process converts signals from the cell surface into mechanical force driving the extension of pseudopodia and lamellipodia. Another important pathway involves the Rho family GTPases where ARPC3 links to actin dynamics cell polarity and the orchestration of actin assembly in response to signaling events within the cell.
Abnormalities in ARPC3 function or expression can influence neurological and immunological conditions. For instance dysregulation of the Arp2/3 complex can enhance susceptibility to diseases like Wiskott-Aldrich syndrome which is tied to impaired signaling and cytoskeletal rearrangement. ARPC3 interacts with proteins related to immune response such as WASP making it significant in the context of immune deficiency disorders. Furthermore mutations affecting actin cytoskeleton dynamics can lead to neurodegenerative diseases highlighting the important impact of ARPC3 and associated Arp2/3 complex proteins in maintaining cellular function.