Abcam, ab38449, Anti-AKT (phospho T308) antibody

Size: 100µg
Anti-AKT (phospho T308) antibody (ab38449) is a rabbit polyclonal antibody detecting AKT in Western Blot, IHC-P . Suitable for Human, Mouse, Rat . - Over 770 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human AKT1 phospho T308 aa 250-350. The exact immunogen used to generate this antibody is proprietary information.P31749,
Specificity:This antibody was made against a peptide directed against the phosphorylated form of AKT1 at T308, but due to a high degree of homology it is predicted to cross react with AKT2 and AKT3 if they are phosphorylated at the corresponding residue.

Product details:
Anti-AKT (phospho T308) antibody (ab38449) is a rabbit polyclonal antibody and is validated for use in IHC-P, WB in human, mouse, rat, recombinant fragment samples.
Anti-AKT (phospho T308) antibody (Anti-RAC antibody; anti-pAKT antibody) (ab38449) has been cited over 779 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-AKT (phospho T308) antibody (ab38449) has high sensitivity and specificity.
Anti-AKT (phospho T308) antibody (ab38449) has 5 independent reviews from customers.
Anti-AKT (phospho T308) antibody (ab38449) specifically detects AKT Phospho-T308 (UniProt ID: P31749; Molecular weight: 56kDa) and is sold in 100 µg selling sizes.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AKT also known as protein kinase B (PKB) is a serine/threonine kinase with a molecular weight of approximately 56 kDa. It plays an important role in signaling pathways regulating cellular processes such as glucose uptake cell survival and growth. AKT is expressed in various tissues including muscle and liver and is often observed using techniques like Western blot. Its kinase activity involves phosphorylation which is essential for its full activation and subsequent biological functions.
Biological function summary
AKT influences cell proliferation and apoptosis. As part of a signaling complex AKT phosphorylation modulates several downstream targets important for cell cycle progression and survival. Its activation depends on upstream kinases like PI3K and PDK1 positioning AKT as a central node in cellular responses. By mediating these processes AKT directly impacts organismal growth and stress response mechanisms.
Pathways
AKT is central to the PI3K/AKT/mTOR and insulin signaling pathways both important for cellular metabolism and growth regulation. It interacts with proteins such as mTOR and GSK3 which are involved in cell growth and glycogen synthesis respectively. These interactions show AKT's pivotal role in maintaining cellular energy balance and metabolic processes.
AKT is frequently linked to cancer and type 2 diabetes. Its abnormal regulation contributes to oncogenesis through uncontrolled cell proliferation and survival. AKT is also associated with insulin resistance in diabetes where it affects glucose metabolism. Through these conditions proteins like PTEN and IRS1 interact with or regulate AKT highlighting its significance in disease states and its potential as a therapeutic target.