Abcam, ab38883, Anti-Tbx6 antibody

Size: 100µg
Rabbit Polyclonal TBX6 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 8 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tbx6 also known as T-box 6 is a transcription factor with a molecular mass of approximately 51 kDa. It belongs to the T-box family which is characterized by a conserved DNA-binding domain. Tbx6 is expressed in the paraxial mesoderm an area of embryonic tissue that eventually forms somites the precursors of skeletal muscle vertebrae and dermis. The expression of Tbx6 is highly regulated during developmental stages indicating its role in embryogenesis and tissue specification.
Biological function summary
Transcription factor Tbx6 plays a significant role in mesoderm specification and somite segmentation. It functions by binding to specific DNA sequences to regulate the expression of target genes essential for somite formation. Tbx6 does not form part of any known protein complex but it interacts with other transcription factors to influence mesoderm differentiation. The precise regulation of Tbx6 activity is important for proper embryonic development ensuring that the cells undertake their correct differentiation pathways.
Pathways
Tbx6 functions in pathways critical for embryonic development including the Wnt and Notch signaling pathways. The Wnt signaling pathway regulates cell fate determination while the Notch signaling controls segmentation during somitogenesis. Tbx6 interacts with proteins such as Wnt3a and RBPJ aligning its activity in these pathways to guide the formation of somites and establish anterior-posterior patterning.
Tbx6 mutations have been associated with congenital vertebral malformations and neural tube defects. These disorders arise due to the disruption of normal somitogenesis and mesoderm development often linked to faulty signaling in the Wnt and Notch pathways. Additionally protein interactions between Tbx6 and RBPJ a component of the Notch pathway highlight their collaborative role in ensuring proper vertebral column formation. Understanding Tbx6's involvement in these pathways provides insights into potential therapeutic targets for such developmental abnormalities.