Abcam, ab40567, Anti-Lamin A + Lamin C antibody [JOL2]

Size: 1mL
Anti-Lamin A + Lamin C antibody [JOL2] (ab40567) is a mouse monoclonal antibody detecting Lamin A + Lamin C in Western Blot, Flow Cytometry, IHC-P . Suitable for African green monkey, Human . - KO validated for confirmed specificity - Over 40 publications - Trusted since 2006
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:JOL2,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human, African green monkey,
Applications:WB, Flow Cyt, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human LMNA. The exact immunogen used to generate this antibody is proprietary information.P02545,
Epitope:This product has been shown to bind to an epitope between amino acids 464-572.,
Specificity:This antibody reacts with both recombinant and native Lamin A and C in humans.

Product details:
What is this antibody validated in?
Anti-Lamin A + Lamin C antibody [JOL2] (ab40567) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in African green monkey, Human samples.
What is the molecular weight of Lamin A + Lamin C?
Anti-Lamin A + Lamin C [JOL2] (ab40567) specifically detects a band for Lamin A + Lamin C (UniProt: P02545) at a molecular weight of 74kDa.
Trusted by the scientific community
Anti-Lamin A + Lamin C [JOL2] (ab40567) was first used in a scientific publication in 2006 and has been cited over 40 times in peer-reviewed journals.
Reviewed by scientists
Anti-Lamin A + Lamin C [JOL2] (ab40567) has over 5 independent reviews from customers.
Specificity confirmed
The specificity of Anti-Lamin A + Lamin C antibody [JOL2] (ab40567) has been confirmed by Western blot testing in LMNA Knockout HAP1 cells.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Purification notes-This product is unpurified., Storage buffer-pH: 7.4Preservative: 0.09% Sodium azideConstituents: Fetal calf serum, 3.16% Tris HCl, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Lamin A and Lamin C also known as lamin A/C are proteins encoded by the LMNA gene. These proteins are key components of the nuclear envelope where they provide structural support and maintain the shape of the nucleus. The lamin A/C molecule has a molecular weight of approximately 60-70 kDa. Expression of lamin A and lamin C occurs predominantly in differentiated cells where these proteins integrate into the nuclear lamina alongside other lamin molecules like lamin B. Lamin A alone sometimes referred to by designations like 4C11 plays a significant role in mechanical support at a molecular level.
Biological function summary
Lamin A/C proteins play a role in maintaining nuclear stability chromosome organization and gene regulation. They are part of a complex network within the nuclear lamina that includes interactions with proteins and DNA. Lamin A with a molecular weight distinct from other lamins participates in assembling this supportive matrix and contributes to DNA maintenance and repair processes. Their interaction with chromatin and gene expression regulation emphasizes their influence on important cellular functions.
Pathways
Lamin A/C proteins engage in the mechanosensory signaling and DNA damage response pathways. They interact with pathways involving the nuclear envelope structure and have connections to proteins like emerin and nuclear actin. Lamin A's role in these pathways supports its involvement in responding to mechanical stress and preserving genomic integrity highlighting its integration with these cellular processes.
Mutations in lamin A/C are linked to disorders such as Hutchinson-Gilford Progeria Syndrome and Emery-Dreifuss Muscular Dystrophy. These conditions highlight the importance of lamin A/C in cellular stability and nuclear integrity. Proteins such as emerin often relate to lamin A/C in these diseases as disruptions to their interactions can lead to compromised nuclear function and disease phenotypes.