Abcam, ab41898, Anti-HNF-4-alpha antibody [K9218]

Size: 100µL
Anti-HNF-4-alpha antibody [K9218] (ab41898) is a mouse monoclonal antibody detecting HNF-4-alpha in Western Blot, Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Rat . - Over 110 publications - Trusted since 2006
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:K9218,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Rat, Human,
Applications:WB, Flow Cyt, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human HNF4A aa 1-100. The exact immunogen used to generate this antibody is proprietary information.P41235

Product details:
Product Specifications
Anti-HNF-4-alpha antibody [K9218] (ab41898) is a mouse monoclonal antibody and is validated for use in Flow Cyt, ICC/IF, IHC-P, WB in human, rat samples.
Anti-HNF-4-alpha antibody [K9218] (ab41898) specifically detects HNF-4-alpha (UniProt ID: P41235; Molecular weight: 53kDa) and is sold in 100 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-HNF-4-alpha antibody [K9218] (ab41898) has high sensitivity and specificity.
Anti-HNF-4-alpha antibody [K9218] (ab41898) has been cited over 110 times in peer reviewed journals and is trusted by the scientific community.
Anti-HNF-4-alpha antibody [K9218] (ab41898) has 8 independent reviews from customers.
This product was changed from ascites to tissue culture supernatant on 3
April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7Preservative: 0.1% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HNF-4-alpha also known as hepatocyte nuclear factor 4-alpha (HNF4A) is a nuclear protein that functions as a transcription factor. It plays an important role in regulating gene expression in the liver kidney and intestine. HNF-4-alpha has a molecular mass of about 54 kDa and is expressed in tissues where it maintains cellular differentiation and metabolic homeostasis. In cellular assays such as ELISA and CHIP detection of HNF-4-alpha provides insights into its expression levels and activity in various biological contexts.
Biological function summary
HNF-4-alpha influences the expression of genes involved in liver and pancreatic function. It acts as a transcriptional regulator that controls fatty acid oxidation gluconeogenesis and insulin secretion. HNF-4-alpha interacts with other proteins to form transcriptional complexes that fine-tune the expression of metabolic enzymes and transport proteins. This protein is key to ensuring that metabolic genes are expressed in a coordinated manner which is essential for maintaining normal metabolic processes.
Pathways
HNF-4-alpha participates in the insulin signaling and lipid metabolism pathways. Through these pathways it interacts with other transcription factors such as HNF-1-alpha and PPAR-alpha which coordinate the regulation of genes involved in glucose metabolism and fatty acid oxidation. HNF-4-alpha plays a critical role by modulating the expression of enzymes like gluconeogenic enzymes in response to metabolic needs which connects it to key metabolic pathways influencing overall energy homeostasis.
HNF-4-alpha is linked to maturity-onset diabetes of the young (MODY1) and certain types of liver diseases. Mutations or dysregulation in HNF-4-alpha can affect the expression of genes essential for insulin production and glucose metabolism leading to disorders like diabetes. Additionally HNF-4-alpha interacts with other proteins and transcription factors implicated in these conditions such as HNF-1-alpha. Understanding the role of HNF-4-alpha in these diseases can provide insights into potential therapeutic targets and intervention strategies.