Abcam, ab45027, Anti-MBD2 + MBD3 antibody [106B691]

Size: 100µg
Mouse Monoclonal MBD2 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human MBD2 aa 200-250.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:106B691,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IF, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MBD2 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.Q9UBB5

Product details:
The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kD and human MBD3 (291 amino acids) is 33 kD.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MBD2 and MBD3 are proteins that belong to the methyl-CpG-binding domain (MBD) family. These proteins recognize and bind to methylated DNA aiding in the interpretation of methylation signals in the genome. MBD2 has a molecular mass of approximately 45 kDa while MBD3 is about 34 kDa. These proteins are expressed in various tissues with notable expression in the brain and embryonic tissues. Furthermore both proteins show a conserved domain that allows them to bind to methylated CpG dinucleotides effectively.
Biological function summary
MBD2 and MBD3 participate in gene silencing and chromatin remodeling. They act as part of the NuRD (nucleosome remodeling and deacetylase) complex which integrates both chromatin remodeling and histone deacetylase activities to regulate transcription. Within this complex MBD2 associates with co-repressor proteins and may directly influence transcriptional repression activities. MBD3 although structurally similar lacks strong methyl-CpG binding and may have a more regulatory role in the NuRD complex.
Pathways
MBD2 and MBD3 influence epigenetic regulatory pathways including those involved in gene expression and genomic imprinting. They interact with pathways that involve DNA methylation and histone modification. Within these pathways MBD2 and MBD3 closely work with proteins such as HDAC1 and HDAC2 which are integral to the deacetylation processes in chromatin remodeling. Their roles in these pathways are important for maintaining cellular identity and regulating developmental processes.
MBD2 and MBD3 have associations with cancer and neurological disorders. In cancer MBD2 can contribute to oncogenesis by aberrantly silencing tumor suppressor genes through DNA methylation. Similarly altered expression of MBD3 has been observed in some cancers potentially impacting cell cycle regulation. Regarding neurological disorders dysregulation of MBD2 and MBD3 affects neuronal development and function linking them to developmental disorders like Rett syndrome. These relationships suggest connections with other regulatory proteins involved in epigenetic controls.