Abcam, ab45150, Anti-c-Myb (phospho S11) antibody [EP769Y]

Size: 100µL / 1mL
Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) is a rabbit monoclonal antibody detecting c-Myb in Western Blot, IP, IHC-P, Dot Blot . Suitable for Human . - Biophysical QC for unrivalled batch-batch consistency - Over 60 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP769Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:Dot, WB, IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Product Specifications
Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) is a rabbit recombinant monoclonal antibody and is validated for use in Dot, IHC-P, IP, WB in human samples.
Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) specifically detects c-Myb Phospho-S11 (UniProt ID: P10242; Molecular weight: 72kDa) and is sold in 100 µL and 1 mL selling sizes.
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) has been cited over 60 times in peer reviewed journals and is trusted by the scientific community.
Related Products
Conjugation-ready, carrier free format available for antibody clone EP769Y -
ab217945
Antibody clone EP769Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 488, Alexa Fluor® 594 (
ab196932
ab197507
ab207025
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The c-Myb protein also known as Myb MYB or c-myb acts mechanically as a transcription factor that regulates the expression of target genes. It plays a role in cellular processes like proliferation differentiation and apoptosis by binding to specific DNA sequences. c-Myb has a molecular mass of roughly 75 kDa. It predominantly expresses in hematopoietic cells where it significantly influences gene transcription activity. Also it appears in some epithelial tissues indicating its versatile role in different cell types.
Biological function summary
The protein serves as a master regulator in the development of various cell types. It is not part of a traditional complex but interacts with other proteins to exert its function such as binding with co-activators or repressors to modulate gene expression. c-Myb controls the transcription of genes that are critical for cell cycle regulation and differentiation especially in the context of blood cell lineages. The protein is particularly influential in the production of red blood cells and white blood cells.
Pathways
C-Myb fits integrally into known hematopoietic signaling cascades and the Wnt signaling pathway. It works alongside other proteins like cyclin-dependent kinase (CDK) family members and transcription factor GATA-1 to modulate lineage-specific gene expression. Through its regulatory actions c-Myb influences the activation of downstream effectors that propagate signals essential for maintaining cellular functions within these pathways. The modulation of these pathways underlines its role in cell fate decisions.
Studies link c-Myb to several forms of leukemia and colon cancer. In leukemia aberrant expression of c-Myb disrupts normal hematopoiesis contributing to the uncontrolled proliferation of immature blood cells. The abnormal interactions between c-Myb and proteins like Bcl-2 can lead to enhanced cellular survival further promoting malignant conditions. In colon cancer c-Myb overexpression influences tumor growth and progression often acting alongside other oncogenic factors to enhance disease severity.