Abcam, ab45174, Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal ACACA antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 82 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP687Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Acetyl Coenzyme A Carboxylase is highly expressed in lipogenic tissues such as liver, adipose, and lactating mammary gland, and its activities are regulated at various levels [Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7515-20.].

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Acetyl Coenzyme A Carboxylase (ACC) also known as acetyl-CoA carboxylase or ACAC is an enzyme that plays an important role in fatty acid metabolism. Mechanically it catalyzes the biotin-dependent carboxylation of acetyl coenzyme A (acetyl-CoA) to produce malonyl-CoA which is an important precursor in the biosynthesis of fatty acids. The molecular weight of ACC is approximately 265 kDa. Humans express this enzyme in multiple tissues such as the liver adipose tissue and mammary glands.
Biological function summary
Acetyl Coenzyme A Carboxylase contributes to fatty acid synthesis and regulation of metabolism. ACC exists in two main isoforms ACC1 which is found mainly in lipogenic tissues and ACC2 which is associated with oxidative tissues. These isoforms form part of larger complexes within the cell interacting with other enzymes and proteins to regulate metabolic processes. ACC also affects the synthesis of long-chain fatty acids by regulating the amount of malonyl-CoA available as a building block.
Pathways
Acetyl Coenzyme A Carboxylase plays a role in the synthesis of fatty acids and their cellular metabolism. This enzyme is a component of the lipogenesis pathway where it transforms acetyl-CoA to malonyl-CoA a step critical for fatty acid elongation. ACC interacts with proteins such as fatty acid synthase to carry out its function within these metabolic pathways. Additionally malonyl-CoA produced by ACC serves as a regulator of carnitine palmitoyltransferase 1 integrating with the fatty acid oxidation pathway.
Alterations in the function of acetyl Coenzyme A Carboxylase link to conditions like obesity and type 2 diabetes. Overexpression of ACC can result in increased fat storage contributing to obesity while its inhibition has been considered a strategy to counter insulin resistance in diabetes. In cancer dysregulation of ACC especially ACC1 can lead to altered lipid synthesis promoting tumor growth. ACC1 interacts with other proteins such as AMP-activated protein kinase (AMPK) which senses energy status and is involved in the regulation of ACC activity thereby influencing these diseases.