Abcam, ab45917, Human CD62L ELISA Kit (Selectin L)

Size: 1 x 96Tests / 2 x 96Tests
Human CD62L ELISA Kit (Selectin L) is a Sandwich (quantitative) ELISA for the measurement of Human CD62L (Selectin L) in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 1 ng/mL,
Range:1.9 - 60 ng/mL,
Assay time:1h 45m,
Assay Platform:Microplate

Product details:
Abcam's CD62L (Selectin L) Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD62L in Human serum, plasma, buffered solutions and cell culture medium.
A monoclonal antibody specific for CD62L has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD62L concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD62L are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD62L present in the samples.
This kit will recognize both endogenous and recombinant Human CD62L.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD62L also known as L-selectin is a cell adhesion molecule with a mass of approximately 74 kDa. It is part of the L-selectin family and is found on the surface of leukocytes including T cells B cells neutrophils and monocytes. Mouse models frequently study CD62L under the name "MEL-14". CD62L plays an important role in mediating the adhesion and rolling of leukocytes on blood vessel endothelial cells during the immune response. Its structure allows it to interact specifically with carbohydrates on endothelial cells facilitating the initial steps of leukocyte extravasation into tissues.
Biological function summary
CD62L is involved in the migration of leukocytes into lymphoid tissues and sites of inflammation. It is not known to be part of a larger protein complex; rather it functions independently on the cell membrane. CD62L also assists in the homing of naive lymphocytes to peripheral lymph nodes. The molecule regulates how immune cells enter tissues through high endothelial venules acting as a critical entry point for leukocyte trafficking.
Pathways
CD62L contributes significantly to the immune trafficking pathway and leukocyte adhesion cascade. It works closely with other adhesion molecules like CD44 and CD54 (ICAM-1) which are important within the context of cellular responses during inflammation. CD62L's function in these pathways ensures that immune cells are properly directed to areas where they are needed most whether that is secondary lymphoid organs or inflamed tissues.
CD62L expression and function have been associated with inflammatory conditions and certain immune-related pathologies such as multiple sclerosis and rheumatoid arthritis. Altered CD62L expression can affect lymphocyte homing potentially playing a role in the pathogenesis of these conditions. The role of CD62L is also linked to proteins like CD44 which can modulate cell interactions and have overlapping functions in disease mechanisms influencing disease progression or resolution.