Abcam, ab46028, Human IL-1 alpha ELISA Kit

Size: 1 x 96Tests / 2 x 96Tests
Human IL-1 alpha ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-1 alpha in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 10 pg/mL,
Range:31.25 - 1000 pg/mL,
Assay time:3h 45m,
Assay Platform:Microplate

Product details:
Abcam's IL-1 alpha Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 alpha in cell culture supernatants, buffered solutions, serum, plasma and other body fluids. This assay will recognize both natural and recombinant Human IL-1a.
A monoclonal antibody specific for IL-1 alpha has been coated to the wells of the microtiter strip plate provided. Samples, including standards of known IL-1 alpha concentrations and unknown are pipetted into these wells. During incubation, binding of IL-1 alpha to the capture antibodies is completed and then any excess unbound analyte is removed by washing. A biotinylated monoclonal antibody specific for IL-1 alpha is then incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1 alpha present in the sample.
This kit will recognize both endogenous and recombinant Human IL-1 alpha.
Get higher sensitivity in only 90 minutes with Human IL-1 alpha ELISA Kit (
ab178008
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Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C