Abcam, ab4674, Anti-GFAP antibody - Astrocyte Marker

Size: 50µL
Anti-GFAP antibody (ab4674) is a chicken polyclonal antibody detecting GFAP in Western Blot, IHC-P, IHC-FrFl, ICC/IF . Suitable for Human, Mouse, Rat . - Over 630 publications - Trusted since 2003
Key facts
Host species:Chicken,
Clonality:Polyclonal,
Isotype:IgY,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:IHC-P, ICC/IF, WB, IHC-FrFlSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human GFAP.P14136

Product details:
Find all reagents to label astrocytes in our "
Astrocytes markers guide
Product Specifications
Anti-GFAP antibody (ab4674) is a chicken polyclonal antibody and is validated for use in ICC/IF, IHC-FrFl, IHC-P, WB in mouse, rat samples.
Anti-GFAP antibody (ab4674) specifically detects GFAP (UniProt ID: P14136; Molecular weight: 50kDa) and is sold in 50 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-GFAP antibody (ab4674) has high sensitivity and specificity.
Anti-GFAP antibody (ab4674) has been cited over 633 times in peer reviewed journals and is trusted by the scientific community.
Anti-GFAP antibody (ab4674) has 59 independent reviews from customers.
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Properties and Storage Information:
Form-Liquid, Purity-IgY fraction, Purification notes-Concentrated IgY fraction of egg yolks., Storage buffer-Preservative: 0.03% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GFAP or Glial Fibrillary Acidic Protein plays a critical role in the structure and function of astrocytes which are star-shaped glial cells in the brain and spinal cord. It is a type III intermediate filament protein with a molecular weight of approximately 50 kDa. GFAP mainly expresses in the central nervous system specifically within astrocytes but you can also find it in non-neuronal cells such as enteric glia. Researchers frequently use GFAP antibodies in laboratory techniques like GFAP western blot and GFAP IHC to study protein expression and localization.
Biological function summary
GFAP contributes to the structural integrity and functional maintenance of the cytoskeleton in astrocytes. This protein integrates into a network of intermediate filaments providing mechanical support to cells. The ability of GFAP to form homodimers and filamentous structures is vital for its function. GFAP antibodies help in highlighting these structures in studies and techniques such as GFAP ELISA utilize these antibodies to quantify protein levels. Through GFAP staining scientists gain insights into the cellular architecture and any alterations under pathological conditions.
Pathways
GFAP plays a significant role within the neurological framework of cell signaling and response to injury. It links to the MAPK signaling pathway which modulates cell growth and differentiation. This protein also interacts with vimentin another intermediate filament protein forming a complex that affects astrocytic responses to environmental changes. These interconnected pathways and interactions highlight GFAP's importance in maintaining homeostasis within the central nervous system.
GFAP anomalies relate to various neurodegenerative conditions and injuries such as Alexander disease and gliosis. Mutations in the GFAP gene form a significant part of Alexander disease pathology where GFAP accumulates abnormally in astrocytes. Its interaction with vimentin aggravates the disease state affecting the astrocytic function. Researchers utilize anti-GFAP antibodies extensively to study disease progression and potential therapeutic targets providing insights into treatments for conditions involving GFAP dysregulation.