Abcam, ab47085, Anti-Nogo A+B antibody

Size: 200µL
Rabbit Polyclonal RTN4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, African green monkey, Rat samples. Cited in 26 publications. Immunogen corresponding to Synthetic Peptide within Human RTN4.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human, African green monkey,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human RTN4. The exact immunogen used to generate this antibody is proprietary information.Q9NQC3,
Specificity:ab47085 recognises Nogo A and Nogo B2 and B1

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-pH: 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nogo A+B also known as Nogo Nogo B or Nogo-B refers to two isoforms of a protein that play a significant role in inhibiting axon regeneration and neurite outgrowth. Nogo A+B are part of the reticulon family of proteins where Nogo A has a molecular mass of approximately 220 kDa while Nogo B is around 55 kDa. These proteins are mainly expressed in the central nervous system specifically in the oligodendrocytes. Nogo A+B can be found on the surface of cells where they interact with specific receptors to carry out their functions.
Biological function summary
Nogo proteins influence cellular functions by acting as inhibitors of neuronal growth and regeneration. Nogo A and B interact with receptors like NgR1 to form a complex that suppresses the regrowth of damaged nerve fibers. It is suggested that this inhibition prevents unwanted and uncontrolled nerve growth maintaining the integrity of the neural architecture. The activity of these proteins is essential for maintaining the stability of cellular functions and structure particularly in neural tissues.
Pathways
These proteins are deeply ingrained in the signaling mechanisms that regulate the inhibition of axon regeneration. The Nogo signaling pathway is critical in modulating nerve growth where Nogo proteins interact with other proteins like RhoA and Rock which are essential in this pathway. This pathway plays an important role alongside the myelin-associated glycoprotein pathway both of which limit axon regeneration and guidance within the nervous system.
Nogo proteins have connections to conditions such as multiple sclerosis and spinal cord injury. In multiple sclerosis the expression of Nogo A limits neural repair contributing to the disease’s progression. Similarly in spinal cord injury increased levels of Nogo proteins inhibit the repair of neurons impeding recovery. These proteins are often studied alongside myelin-associated inhibitors such as MAG (Myelin-Associated Glycoprotein) which also plays a part in limiting neural regrowth further implicating Nogo proteins in these disorders.