Abcam, ab4797, Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody

Size: 50µL
Rabbit Polyclonal GSK3 alpha phospho Y216 antibody. Suitable for WB and reacts with Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Mouse Glycogen synthase kinase-3 alpha.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Mouse Glycogen synthase kinase-3 alpha. The exact immunogen used to generate this antibody is proprietary information.Q2NL51

Product details:
Glycogen synthase kinase-3 (GSK 3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK 3A) and beta, show a high degree of amino acid homology. GSK 3B is involved in energy metabolism, neuronal cell development, and body pattern formation.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified from rabbit serum by epitope specific affinity chromatography. Any reactivity towards the non-tyrosine phosphorylated GSK 3 alpha + beta protein has been eliminated through a series of preabsorption steps., Storage buffer-pH: 7.3Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The GSK3 protein known also as glycogen synthase kinase 3 has two isoforms: GSK3 alpha and GSK3 beta. Both isoforms have distinct yet significant mechanical roles. GSK3 alpha with a molecular weight of approximately 51 kDa and GSK3 beta weighing about 47 kDa act as serine/threonine kinases. These proteins are expressed in various tissues including the brain pancreas and liver. GSK3 is involved in the regulation of numerous cellular processes by phosphorylating its substrates impacting various cellular activities.
Biological function summary
GSK3 is integral in multiple signaling pathways and participates in the regulation of inflammation cell cycle and apoptosis. As part of the larger complex of signaling proteins GSK3 impacts the modulation of transcription factors and influences cellular differentiation and proliferation. The protein's kinase activity is necessary for maintaining cellular homeostasis and ensuring proper response to external signals.
Pathways
GSK3 influences critical pathways like Wnt and PI3K/Akt. Within the Wnt pathway GSK3 targets important proteins such as β-catenin where it plays an important role in controlling gene expression related to cell growth. In the PI3K/Akt pathway GSK3 acts as an important regulatory protein affecting insulin signaling and energy metabolism. Interactions with other kinases and transcription factors allow GSK3 to integrate various signaling cues that dictate cellular responses.
GSK3 has associations with neurodegenerative diseases and diabetes. In Alzheimer's disease GSK3 is linked to the formation of tau protein tangles contributing to neuronal dysfunction. Moreover in the context of diabetes GSK3 is involved in the insulin signaling pathway where dysregulation can lead to insulin resistance. It interacts with proteins such as tau in neurodegeneration and insulin receptor substrates in diabetes showing its significant role in these pathological conditions.