Abcam, ab48187, Anti-IRE1 (phospho S724) antibody

Size: 100µL
Anti-IRE1 (phospho S724) antibody (ab48187) is a rabbit polyclonal antibody detecting IRE1 in Western Blot, IHC-P, ELISA . Suitable for Human, Mouse, . - Over 280 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, ELISA, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ERN1 phospho S724. The exact immunogen used to generate this antibody is proprietary information.O75460,
Specificity:Stimulation is generally required to allow detection of the target protein. Please see product images for recommended treatment conditions and controls. If you are testing Human samples in Western Blot, please also see ab124945.

Product details:
Product Specifications
Anti-IRE1 (phospho S724) antibody (ab48187) is a rabbit polyclonal antibody and is validated for use in ELISA, IHC-P, WB in human, mouse, recombinant fragment samples.
Anti-IRE1 (phospho S724) antibody (ab48187) specifically detects IRE1 Phospho-S724 (UniProt ID: O75460; Molecular weight: 108kDa) and is sold in 100 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-IRE1 (phospho S724) antibody (ab48187) has high sensitivity and specificity.
Anti-IRE1 (phospho S724) antibody (ab48187) has been cited over 283 times in peer reviewed journals and is trusted by the scientific community.
Anti-IRE1 (phospho S724) antibody (ab48187) has 19 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.025% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The inositol-requiring enzyme 1 (IRE1) also known as ERN1 or IRE1 alpha is a critical endoplasmic reticulum (ER) stress sensor. It has a molecular weight of approximately 110 kDa. IRE1 is expressed in various cell types and tissues particularly in those subject to a high degree of protein synthesis such as the liver pancreas and secretory cells. This protein plays a dual role as both a RNase and a kinase which enables it to respond swiftly to misfolded proteins accumulating in the ER.
Biological function summary
IRE1 is an important regulator in the unfolded protein response (UPR) a cellular reaction to stress in the ER. It operates as part of a complex mechanism facilitating the splicing of X-box binding protein 1 (XBP1) mRNA which results in the production of a potent transcription factor. IRE1 activity helps in restoring normal function of the cell by upregulating genes involved in protein folding secretion and degradation. Its actions are important for maintaining cellular homeostasis during stressful conditions.
Pathways
IRE1 is an integral component of the UPR pathway which works to alleviate ER stress. It interacts closely with other UPR transducers such as activating transcription factor 6 (ATF6) and protein kinase RNA-like ER kinase (PERK). IRE1 connects with the XBP1 pathway facilitating adaptive responses that enhance protein-folding capacity lipid biosynthesis and ER-associated degradation. Altogether these pathways mediate cell survival or apoptosis depending on the severity of the stress.
IRE1 has significant involvement in conditions like diabetes and cancer. In the context of diabetes improper UPR signaling due to chronic ER stress leads to insulin resistance and pancreatic beta-cell dysfunction. In cancer IRE1 modulates tumor microenvironment and promotes cancer cell survival under hypoxic conditions. The XBP1 pathway linked with IRE1 also plays a substantial role in these diseases by influencing cell proliferation and apoptosis. Understanding the mechanisms of IRE1 in these conditions might provide therapeutic insights.