Abcam, ab48389, Anti-Detyrosinated alpha Tubulin antibody - Loading Control

Size: 100µg
Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker (ab48389) is a rabbit polyclonal antibody detecting Detyrosinated alpha Tubulin in Western Blot . Suitable for Human . - Over 80 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human TUBA4A. The exact immunogen used to generate this antibody is proprietary information.P68366

Product details:
What is this antibody validated in?
Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker (ab48389) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human samples.
What is the molecular weight of Detyrosinated alpha Tubulin?
Anti-Detyrosinated alpha Tubulin - Microtubule Marker (ab48389) specifically detects a band for Detyrosinated alpha Tubulin (UniProt: Q71U36) at a molecular weight of 50kDa.
Trusted by the scientific community
Anti-Detyrosinated alpha Tubulin - Microtubule Marker (ab48389) was first used in a scientific publication in 2007 and has been cited over 80 times in peer-reviewed journals.
Reviewed by scientists
Anti-Detyrosinated alpha Tubulin - Microtubule Marker (ab48389) has over 10 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Detyrosinated alpha Tubulin also known as detyrosinated tubulin or alpha tubulin is a modified form of the alpha subunit of tubulin. This protein undergoes detyrosination which entails removing the terminal tyrosine residue. Detyrosinated alpha Tubulin has an approximate molecular mass of 50 kDa. It localizes mainly in the cytoplasm and plays an important role in the stability and function of microtubules which are key components of the cytoskeleton. These modifications regulate microtubules' dynamic properties impacting cellular processes such as intracellular transport and cell division.
Biological function summary
Detyrosinated alpha Tubulin impacts important cellular functions. It is an integral part of the microtubule network. Microtubules provide structural support to the cell aiding in intracellular transport and cell shape maintenance. The detyrosination of alpha tubulin contributes to the regulation of microtubule stability which is essential for accurate chromosome segregation during mitosis. Furthermore the detyrosination influences interactions with microtubule-associated proteins and motor proteins affecting their binding affinity and activity.
Pathways
Detyrosinated alpha Tubulin is significant in cellular signaling and transport. It is an important part of the microtubule-dependent transport pathways that facilitate the movement of cellular components including vesicles and organelles. The detyrosination of alpha tubulin influences its interaction with motor proteins such as kinesins and dyneins which are responsible for directional transport along microtubules. Additionally alterations in tubulin detyrosination affect the Wnt signaling pathway impacting cellular processes such as proliferation and differentiation.
Alterations in the detyrosination of alpha Tubulin have links to neurodegenerative diseases and cancer. In neurodegenerative disorders abnormal detyrosination can disrupt axonal transport contributing to diseases like Alzheimer's. In cancer increased levels of detyrosinated alpha Tubulin can enhance cell invasiveness and metastasis. The dysregulation of tubulin detyrosination affects cellular interactions with proteins such as tau in neurodegenerative disorders and beta-catenin in cancer implicating these proteins in disease progression.