Abcam, ab49033, Recombinant Hepatitis C Virus genotype 1a NS5 protein

Size: 100µg
Recombinant Hepatitis C Virus genotype 1a NS5 protein is a Hepatitis C virus (isolate 1) Fragment protein, in the 2212 to 2313 aa range, expressed in Escherichia coli, with >95%, suitable for ELISA, WB, Flow Cyt.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Escherichia coli,
Tags:GST tag N-Terminus,
Applications:ELISA, Flow Cyt, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P26664,
Animal free:No,
Carrier free:No,
Species:Hepatitis C virus (isolate 1),
Storage buffer:pH: 7.2 - 7.6Constituents: 50% Glycerol (glycerin, glycerine), 9% Urea, 0.395% Tris HCl, 0.2% Triton X-100

Product details:
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Hepatitis C Virus genotype 1a NS5 also called non-structural protein 5 plays an important role in viral replication. The NS5 protein encompasses two important domains: NS5A and NS5B with a molecular weight of roughly 100 kDa. It is expressed in infected host cells and its functional activities are primarily associated with the hepatitis C replication complex. This complex resides on the endoplasmic reticulum membranes within the host cell cytoplasm which facilitates the efficient replication of the viral RNA.
Biological function summary
The NS5 protein is indispensable for the formation and stabilization of the replication complex. It acts as a component of a larger viral replication ensemble working alongside other non-structural proteins. NS5A is implicated in modulating interferon response and regulating lipid metabolism whereas NS5B serves as the RNA-dependent RNA polymerase essential for viral RNA synthesis. These functions make NS5 pivotal in enabling the virus to maintain its lifecycle within the host cell.
Pathways
NS5A and NS5B of the hepatitis C virus interact with cellular pathways that control replication and virulence. The NS5A protein interacts within the PI3K/AKT/mTOR signaling pathway influencing cell proliferation and apoptosis. Moreover NS5B as the RNA polymerase is at the heart of the viral replication machinery and shares functional interactions with the host's cyclophilin A which can influence the RNA binding process. These interactions make the components of genotype 1an important for understanding its replication and persistence.
Hepatitis C Virus genotype 1a NS5 is closely linked to chronic hepatitis C infection and liver cirrhosis. The replication efficiency attributed to NS5 highlights its importance in the persistence of the virus within the liver leading to long-term liver damage. The interaction of NS5A with the host's immune response proteins such as interferon-alpha contributes to the virus's ability to evade immune detection and clearance. This relationship with key immune players complicates the pathogenesis and treatment of Hepatitis C infections.