Abcam, ab49873, Anti-Glutamine Synthetase antibody

Size: 50µL
Anti-Glutamine Synthetase antibody (ab49873) is a rabbit polyclonal antibody detecting Glutamine Synthetase in Western Blot . Suitable for Rat . - Over 70 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Mouse Glul aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P15105

Product details:
What is this antibody validated in?
Anti-Glutamine Synthetase antibody (ab49873) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Rat samples.
What is the molecular weight of Glutamine Synthetase?
Anti-Glutamine Synthetase (ab49873) specifically detects a band for Glutamine Synthetase (UniProt: P15104) at a molecular weight of 42kDa.
Trusted by the scientific community
Anti-Glutamine Synthetase (ab49873) was first used in a scientific publication in 2007 and has been cited over 70 times in peer-reviewed journals.
Reviewed by scientists
Anti-Glutamine Synthetase (ab49873) has over 10 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Ion exchange chromatography, Purification notes-Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins., Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glutamine synthetase also known as glutamine s synthetase or glnA is an enzyme that catalyzes the ATP-dependent conversion of glutamate and ammonia into glutamine. This reaction plays an important role in nitrogen metabolism. Glutamine synthetase has a molecular weight of approximately 620 kDa and forms a multimeric structure commonly seen in bacteria plants and animal tissues with significant expression in the brain liver and kidneys.
Biological function summary
This enzyme supports the detoxification of ammonia by incorporating it into glutamine an essential amino acid and nitrogen donor. Glutamine synthetase operates independently rather than as part of a larger protein complex. It assists in maintaining cellular nitrogen balance and facilitates the synthesis of proteins and other nitrogen-containing molecules. Glutamine peptides serve vital roles in cellular processes underlining the significance of their synthesis.
Pathways
Glutamine synthetase integrates into the glutamate and glutamine cycle between neurons and glial cells highlighting its part in neurotransmitter metabolism. It also features prominently in the urea cycle influencing nitrogen disposal in organisms. Glutamine synthetase interacts with glutaminase which assists in transforming glutamine back to glutamate maintaining a balance of nitrogenous compounds within these pathways.
Glutamine synthetase abnormalities link to hepatic encephalopathy and neurodegenerative disorders such as Alzheimer's disease. Altered enzyme expression contributes to increased ammonia levels adversely affecting brain function. In Alzheimer's disease connections with tau and amyloid-beta proteins suggest a link between glutamine synthetase activity and neurotoxic events. Understanding these interactions may offer insights into therapeutic approaches for these conditions.