Abcam, ab49900, HRP Anti-beta Actin antibody [AC-15] - Loading Control

Size: 50µL
Anti-beta Actin antibody [AC-15] - HRP conjugated (ab49900) is a mouse monoclonal antibody detecting beta Actin in Western Blot . Suitable for African green monkey, Cow, Dog, Hamster, Human, Mouse, Rat . - Over 450 publications - Trusted since 2007
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:AC-15,
Isotype:IgG1,
Conjugation:HRP,
Carrier free:No,
Reacts with:Hamster, Mouse, Rat, Cow, Dog, Human, African green monkey,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ACTB aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P60709

Product details:
HRP Anti-beta Actin antibody [AC-15] (ab49900) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in WB in african green monkey, cow, dog, hamster, human, mouse, rat samples.
HRP Anti-beta Actin antibody [AC-15] (ab49900) has been cited over 457 times in peer reviewed journals and is trusted by the scientific community.
HRP Anti-beta Actin antibody [AC-15] (ab49900) has 10 independent reviews from customers.
HRP Anti-beta Actin antibody [AC-15] (ab49900) specifically detects beta Actin (UniProt ID: P60709; Molecular weight: 42kDa) and is sold in a 50 uL selling size.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.01% Thimerosal (merthiolate)Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.
Biological function summary
Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.
Pathways
Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.
Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.