Abcam, ab49999, Anti-iNOS antibody [NOS-IN]

Size: 50µL
Anti-iNOS antibody [NOS-IN] (ab49999) is a mouse monoclonal antibody detecting iNOS in Western Blot, ICC/IF . Suitable for Mouse . - Over 80 publications - Trusted since 2007
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:NOS-IN,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Mouse Nos2 aa 1100 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P29477,
Specificity:This antibody reacts specifically with inducible-type nitricoxide synthase (iNOS, also termed macNOS and mNOS). It does not react with NOS derived from brain.

Product details:
What is this antibody validated in?
Anti-iNOS antibody [NOS-IN] (ab49999) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.
What is the molecular weight of iNOS?
Anti-iNOS [NOS-IN] (ab49999) specifically detects a band for iNOS (UniProt: P35228) at a molecular weight of 131kDa.
Trusted by the scientific community
Anti-iNOS [NOS-IN] (ab49999) was first used in a scientific publication in 2007 and has been cited over 80 times in peer-reviewed journals.
Reviewed by scientists
Anti-iNOS [NOS-IN] (ab49999) has over 5 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification notes-This antibody is purified from hybridoma cell culture., Storage buffer-pH: 7.4Preservative: 0.087% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Inducible Nitric Oxide Synthase commonly referred to as iNOS or NOS2 is an important enzyme in the production of nitric oxide (NO) from L-arginine. This enzyme has a molecular weight of approximately 130 kDa and is expressed in various cell types including macrophages and hepatocytes. iNOS becomes active in response to inflammatory stimuli and can produce large amounts of NO for extended periods. Western blotting and iNOS ELISA kits are frequently used methods to detect and quantify the presence and activity of iNOS in different biological samples.
Biological function summary
INOS plays a significant role in the immune system by participating in the defense against pathogens. The enzyme is part of a molecular complex that generates NO which acts as a signaling molecule and has antimicrobial properties. iNOS activity is induced during immune responses and contributes to the elimination of bacteria and viruses through the production of reactive nitrogen species. Its expression is tightly regulated by cytokines and other signaling molecules that modulate the extent of its action.
Pathways
INOS integrates into the nitric oxide signaling pathway and is closely associated with inflammatory pathways. NO serves as a messenger within the nitric oxide signaling pathway facilitating processes like vasodilation and neurotransmission. iNOS works alongside other proteins such as cytokines like interferon-gamma that elevate its activity during inflammation and immune responses. The regulation of iNOS expression and activity plays an essential role in maintaining immune homeostasis and ensuring proper response to infections.
INOS is linked to conditions characterized by excessive inflammation such as rheumatoid arthritis and sepsis. In these diseases overproduction of nitric oxide by iNOS can lead to tissue damage and exacerbate symptoms. Additionally iNOS is associated with the protein TNF-alpha as its expression is often driven by inflammatory mediators during disease states. Understanding the role of iNOS in such conditions provides insights into potential therapeutic targets for managing inflammatory diseases.