Abcam, ab500, ChIP Kit

Size: 1Kit
Chromatin immunoprecipitation (ChIP) kit (ab500) allows the mapping of target proteins/histone modifications to specific loci in the genome of interest. Suitable for mammalian suspension and adherent cells. - Proven performance: cited in over 165 publications - Includes positive control Histone H3 antibody (ab1791) - qPCR-ready DNA purification
Key facts
Sample types:Suspension cells, Adherent cells

Product details:
ChIP kit ab500 provides a protocol and reagents for running ChIP assays including:
- cell lysis and chromatin extraction
- chromatin shearing and DNA fragment length analysis
- immunoprecipitation and DNA purification
DNA produced using the kit can be analyzed using qPCR.
The kit has been validated for ChIP assays with mammalian samples.
Chinese protocol available.
See protocols section below.
This kit uses Protein A sepharose beads for antibody pulldown. Seeprotocol booklet for Protein A and Protein G binding affinities with antibodies from commonly used species. For other species of antibody, consult the table in the protocol booklet.
Species raised in
Isotype
Protein A binding affinity
Protein G binding affinity
Rabbit
All isotypes
All isotypes
Use anti-mouse IgM
ChIP assay products and guides
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REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols