Abcam, ab50036, Recombinant human TGF beta 1 protein (Active)

Size: 2µg / 10µg
Recombinant human TGF beta 1 protein (Active) is a Human Full Length TGF-beta 1 protein, in the 279 to 390 aa range with >98% purity, < 1 EU/µg endotoxin level and suitable for Cell Culture, Functional studies, western blot, HPLC and SDS-PAGE. The predicted molecular weight of ab50036 recombinant protein is 25 kDa. - Save time and ensure accurate results - using our recombinant TGF-beta 1 cytokine protein - Optimal protein bioactivity, stability and reproducibility - Available in different sizes to fit your experimental needs - Cited in over 60 publications
Key facts
Purity:>98% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:HEK 293 cells,
Tags:Tag free,
Applications:SDS-PAGE, FuncS, Cell Culture, WB, HPLCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The ED50, as determined by TGF-beta1's ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells, is ≤ 0.05 ng/ml), corresponding to a specific activity of ≥ 2 x 107 units/mg.,
Accession:P01137,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:reconstitute with 10 mM citric acid, pH 3.0 at 1mg/mL

Product details:
Ensure the validity of your result using our Recombinant TGF beta 1 protein ab50036 as a control. ab50036 can be used as a positive control in SDS-PAGE and western blot assays.
Check out our Western blot protocol for more information
TGF-beta is secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix.
The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1.
TGF-β1 is the most abundant isoform secreted by almost every cell type.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle