Abcam, ab50733, Anti-EIF2S1 antibody [EIF2S1A2B8]

Size: 100µg
Mouse Monoclonal EIF2S1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EIF2S1.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:EIF2S1A2B8,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human EIF2S1.P05198

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-ab50733 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum., Storage buffer-pH: 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 1% BSA, 0.812% Sodium chloride, 0.1136% Disodium hydrogenorthophosphate, 0.0225% Potassium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EIF2S1 also called eIF2α is a subunit of the eukaryotic translation initiation factor 2 (eIF2) complex. This protein plays an important role in the initiation of protein synthesis by regulating the binding of the initiator methionyl-tRNA to the 40S ribosomal subunit. Its molecular weight is approximately 36 kDa. EIF2S1 is widely expressed in various tissues providing a fundamental function in protein biosynthesis across different cell types.
Biological function summary
EIF2α acts as a regulatory component of the eIF2 complex which is central to initiating translation in eukaryotic cells. Phosphorylation of eIF2α leads to inhibition of the exchange of GDP to GTP on eIF2 effectively reducing global protein synthesis while allowing the translation of specific mRNAs involved in stress responses. The eIF2α forms part of the eIF2 complex working closely with other subunits to exert its regulatory functions.
Pathways
EIF2α is involved in the integrated stress response (ISR) and other protein synthesis pathways. The phosphorylation state of eIF2α is important in ISR linking it to proteins such as PERK and GCN2 which respond to various stress signals. Phospho-eIF2α plays a significant role in modulating the translation response to cellular stress ensuring cells can adapt or initiate apoptosis depending on the conditions.
EIF2α connects to diseases like Alzheimer's and diabetes. Alterations in phosphorylation of eIF2α are linked to endoplasmic reticulum stress in Alzheimer's affecting protein homeostasis. Connecting to diabetes dysregulated eIF2α signaling interferes with insulin synthesis and secretion. Proteins like ATF4 also intersect with eIF2α in these diseases highlighting eIF2α's involvement in critical pathological processes.